Posted by admin on May 30, 2017 in |
Mangiferin content in aqueous extracts of 30 varieties of Mangifera indica leaves collected from Gujarat state of Western India has been estimated during this study, using a simple reverse phase HPLC-DAD analysis method. The proposed method has been validated according to ICH guidelines in terms of linearity, accuracy, intraday and interday precision, limit of detection and limit of quantitation. The calibration graph was found to be linear over a concentration range of 20-120 µg/mL, with a regression coefficient of 0.9943. Determination of accuracy by standard addition method at three different concentration levels returned a mean recovery value of 97.64%. Average intraday and interday precision values were found to be less than...
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Posted by admin on May 30, 2017 in |
Objective: The study was designed to explore the antibacterial, antifungal and antioxidant potential of Jatropha curcas Linn. (J. curcas) stem bark extract. Materials and Methods: The chemotherapeutic action was measured in the crude extract using agar well diffusion method. The antibacterial and antifungal activities of different extracts were tested against two gram-positive and nine gram negative human pathogenic bacteria using agar well diffusion method. Area of the reticence of crude extracts was matched with that of various antibiotics like ampicillin, streptomycin for antibacterial property and fluconazole for antifungal activity. The antioxidant and free radical scavenging activities of different extracts of the Jatropha curcas were also investigated against 2,2 Diphenyl –1- picrylhydrazyl (DPPH), 2,2, Azion-bis- (3- ethylbenzothia-zoline-96- sulphonic acid) (ABTS), superoxide anion (O-2) and nitric oxide (NO) using spectroscopic absorption methods. The data were statistically analyzed by ANOVA, arranged in completely randomized design (CRD) in the factorial arrangement having four replications. Results: The average inhibition zone for methanol extract (25.31mm) was establish to be extra valuable than the average inhibition...
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Posted by admin on May 30, 2017 in |
In this study antioxidant activity was performed by DPPH (1,1 diphenyl-2-picrylhydrazyl) radical scavenging method for Tribulus terrestris L. whole plant methanolic extraction fractionation with toluene and n-butanol in succession. The obtained fractions were concentrated under reduced pressure to yield corresponding antioxidant activity. The IC50 concentration for the standard, ascorbic acid and BF-TTME was found to be 0.085 and 4.5 μg/ml...
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Posted by admin on May 30, 2017 in |
The present study was designed to judge the in vitro antibacterial activity and comparison of Ocimum basilicum and Cymbopogon martinii. From the folklore claim and ethnobotanical review, the plant Ocimum basilicum and Cymbopogon martinii leaves have been found to contain terpenes and terpenoids i.e. essential oil, and the plants are used in indigenous system of medicine for the treatment of bacterial infections. It is generally considered that compounds produced naturally rather than synthetically, will be biodegraded more easily and be therefore environmentally more acceptable. Thus, natural drugs which are used to treat various diseases antibacterial, antiviral, antifungal, antioxidants, cytotoxic and nutrients gained importance in the recent years, and their use and positive image among consumers are spreading. In recent years multiple drug resistance in both human and plant pathogenic microorganisms have been developed due to the indiscriminate use of commercial antimicrobial drugs commonly used in the treatment of infectious diseases. To find new therapeutic agents, plants that have antibacterial (antimicrobial) activity have attracted the attention of researchers. The antibacterial...
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Posted by admin on May 30, 2017 in |
Acmella oleracea (L.) R.K. Jansen (family Asteraceae) is an interesting plant because of its wide range of medicinal and culinary uses. It has been traditionally used in the treatments of anemia, body ache, cancer, gastric ulcer and infections, gingivitis, gout, inflammations, laceration, malaria, stammering, and worm infection (helminthiasis). Different extracts of the plants were prepared, and the methanol extract showed the highest number of bioactive compounds, including carbohydrates, phytosterols, and tannins. The antioxidant activity was estimated from the rate of scavenging of the free radical, 2,2-diphenyl-1-picrylhydrazyl (DPPH). The plant extract showed similar concentration-dependent scavenging activity as that of butylated hydroxytoluene (BHT). The total antioxidant activity was estimated against ascorbic acid, and it showed a higher value than BHT. The methanol extract was treated with two Gram-negative bacteria Pseudomonas aeuroginosa and Escherichia coli, and two gram-positive bacteria Staphylococcus aureus and Bacillus subtilis. However, no antibacterial activity was observed. Nonetheless, the study provides important information on the basic pharmacological properties of the...
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