Posted by admin on Oct 1, 2014 in |
Oxidative stress has been implicated in various pathological diseases like neurodegenerative disorders, stroke, cancer, inflammation, atherosclerosis, etc., which upshots when the production of reactive oxygen species overwhelms the antioxidant defense mechanism. Nowadays, development of drugs with antioxidant potential gained utmost importance therapeutically to treat many pathological diseases. Plant-derived anti-oxidants were proven to be more effective than synthetic antioxidants. With this as focus, the present study investigated the antioxidant potential of punicalagin (PG) in an in-vitro system of goat liver slices imperiled to oxidative stress. Precision cut goat liver slices are used as an in-vitro model for the evaluation of anti-oxidant activity because it crafts in-vivo tissue environment and helps in curtailing the use of intact animals. In our study, hydrogen peroxide is used as an oxidant to create oxidative stress to the liver slices. Hydrogen peroxide treated liver slices showed a significant decrease in both enzymatic and non-enzymatic antioxidant levels as compared with normal liver slices. PG treated liver slices showed significant improvement in both enzymatic and non-enzymatic antioxidant...
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Posted by admin on Oct 1, 2014 in |
The present study was conducted to explore the antioxidant and radical scavenging activities of essential oil extracted from the leaves of plants of family Lamiaceae from Morni hills in the north-eastern part of the state of Haryana, India. Ten plants- Hyptis suaveolens, Mentha longifolia, M. pulegium, Ocimum basilicum, Plectranthus incanus, P. striatus, Pogostemon benghalensis, Rabdosia rugosa, Salvia plebeia and Scutellaria angulosa of the family Lamiaceae were explored from the area and the essential oil from their leaves was extracted. The maximum oil yield was found in leaves of O. basilicum, while the oil yield was the minimum in leaves of P. incanus. The density of oil was found to be the maximum in H. suaveolens, while it was the minimum in P. incanus and S. angulosa. The essential oils of all the plants exhibited DPPH radical scavenging activity; however, it was best exhibited by S. angulosa (with least IC50 value) in comparison to standard butylated hydroxytoluene (BHT). Further, the oil also exhibited total antioxidant activity, which was found to...
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Posted by admin on Oct 1, 2014 in |
The study was carried out to assess the analgesic, anti-inflammatory, CNS depressant and anti-diarrhoeal activity of methanolic extract of Nephelium lappaceum L. seeds (MNLS). Analgesic activity was evaluated using acetic acid-induced writhing and formalin-induced licking paw test in mice. Anti-inflammatory effect was studied using the carrageenan-induced inflammatory method. Hole cross and open field models were used to evaluate CNS depressant activity whereas castor oil- induced diarrhoeal model was employed for testing anti-diarrhoeal activity. In the analgesic activity, MNLS at a dose of 500 mg/kg exhibited potent (51.27%) activity against acetic acid-induced pain in mice whereas indomethacin (10 mg/kg) displayed 58.86% inhibition. Furthermore, the extracts showed 50.87% and 57.60% inhibition of the formalin-induced paw licking in mice at a dose of 250 and 500 mg/kg b.w. respectively and indomethacin produced 70.72% inhibition. In the carrageenan-induced paw edema test, the extract showed a significant (p<0.05) inhibition of paw edema after 30 min to a 4th h of the study period. In hole cross and open field tests, maximum 88.09% and 85.94%...
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Posted by admin on Sep 1, 2014 in |
The antibacterial activity and phytochemical screening of red algae Amphiroa anceps were using three different solvents like aqueous, methanol and ethyl acetate extracts. The phytochemical screening of macroalgae showed the presence of flavonoids and terpenoids in methanol and ethyl acetate extract. The aqueous extract of Amphiroa anceps observed only tannins. The eight human pathogenic bacteria like Escherichia coli, Staphylococcus aureus, Proteus vulgaris, Klebsiella sp, Pseudomonous aeruginosa, Spreptococcus sps, Enterobacter sps and Neisseria sps used for the antibacterial activity. The highest zone of inhibition was recorded in ethyl acetate extract of the Amphiroa ance against the bacteria Enterobacter...
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Posted by admin on Sep 1, 2014 in |
Pupalia lappacea Juss belongs to the family Amaranthaceae useful in the bone fracture treatment, antimalarial, pyrexia, wounds, cough, toothache and boils. The present investigation was aimed to identify the phytocompounds by GC-MS, investigate phytochemically and to screen anti-inflammatory and antioxidant property by in-vitro methods. Bioactive compounds were identified using GC- MS; functional groups were identified using FT-IR, phytochemical estimation was done for the phenolics, flavonoids, tannins, and alkaloids, in-vitro anti-inflammatory activity by heat-induced hemolytic and albumin denaturation method, in-vitro antioxidant by DPPH, SOD, NO, PPO and CAT methods. GC-MS analysis of the methanolic extract confirmed the presence of bioactive principles like phenols, tetradecanoic acid, pentadecanoic acid, Eicosatetraenoic acid, Hexatriacontane, eicosapentaenoic acid, and chloroundecane. Methanolic extract of aerial parts of Pupalia lappacea was estimated for the presence of secondary metabolites and screened for in-vitro anti-inflammatory and antioxidant effects. Aerial parts were extracted and estimated for total phenolics 248.19 ± 4.84, flavonoids 123.47 ± 0.19, tannins 57.12 ± 2.45 and alkaloids 69.29 ± 1.84 quantitatively reaction equivalent per gram of the...
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