Posted by admin on Sep 30, 2021 in |
This study deals with the Pharmacognostical physicochemical and phytochemical evaluation of the whole plant of Piper longum L. Pharmacognostical studies includes macroscopic, microscopic, and physicochemical evaluation. Physicochemical constants showed the values of total ash (10%), acid insoluble ash (1%), water-soluble ash (32%), sulphated ash (22%), nitrated ash (18.5%), hot extraction (11.2%), water-soluble extractive (4.8%), ethanol-soluble extractive (12.4%), ether soluble extractive (5.6%), loss on drying (29%), swelling index (0.33) and mucilage content (0.8%) w/w. The preliminary phytochemical screening of the alcoholic extract showed alkaloids, phenolic compounds, tannins, mucilage, starch, steroid, and flavanoids. This study helps to development of different standard parameters of the plant, which help the plant identification and...
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Posted by admin on Sep 30, 2021 in |
Objective: To investigate the Pharmacognostic and in-vitro Antioxidant, Anti-inflammatory & Anti-diabetic Activity of the flower of the Himalayan Rhododendron Arboreum. Methods: The collected plant flowers of Rhododendron Arboreum were cleaned with distilled water and air-dried at room temperature under shade (25-27 °C) and reduced to the appropriate size of the powder. The powdered plant materials are further extracted by cold maceration with hydroalcoholic as well as the aqueous solvent. The filtrates obtained from the successive maceration were concentrated under reduced pressure using a rotary evaporator followed by hot air set at 40 °C. The dry extract obtained was harvested, and the dried extract was transferred into airtight bottles and stored in a refrigerator at -4 °C. The powdered flowers of Rhododendron Arboreum and hydroalcoholic and aqueous extract were standardized via pharmacognostic parameters. Phytochemical screening of Hydro-alcoholic and aqueous extract of Rhododendron Arboreum revealed the presence of tannins, flavonoids, phenol, triterpenes, Steroids & carbohydrates. Different flowers extract of Rhododendron Arboreum accessed for in vitro antioxidant potential by using the DPPH...
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Posted by admin on Jul 31, 2021 in |
The demand for today’s chemistry is finding an efficient alternative to synthetic indicators. Acid-base titration was conducted using natural indicators in the present research work. The natural indicator is prepared from Jasminum sambac, the most commonly occurring plant. The ethanolic flower extract was used as a natural indicator. For acid-base titration, Hydrochloric acid (HCl) as strong acid and Acetic acid (CH3COOH) used as a weak acid, and Sodium hydroxide (NaOH) were chosen as a strong base. Successful results were achieved when it was examined by using standard synthetic indicators. Titration shows colour change at the point of equivalence. Thus, for acid-base titration at any dilution, natural indicators from flowers can be used. Titration with natural indicator from ethanolic flower extract of Jasminum sambac has not yet been performed by any research team. It is very simple and economical to use ethanolic flower extract of Jasminum sambac as an indicator as it provides the same outcome accuracy as that provided by the synthetic...
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Posted by admin on Jun 30, 2021 in |
Objective: Ariel part of Opuntia elatior (Cactaceae) has been used in folklore health systems to treat diabetes in America, Mexico, and India. However, the anti-diabetic potential of cladodes of this medicinal plant is not scientifically validated and authenticated. The present study aimed to evaluate in-vitro and in-vivo anti-oxidant and anti-diabetic potential of methanol extracts and solvent fractions of O. elatior cladodes. Methods: The methanol extracts and solvent fractions of Opuntia elatior cladodes were evaluated at different concentrations (12.5-400 µg/ml) for anti-oxidant activity by using DPPH method and different doses (200 and 400 mg/kg body weight) for anti-diabetic potentials in staptozotocin induced diabetic albino rats. The extracts were administered for three weeks in different groups. Results: The acute toxicity study of Opuntia elatior cladodes extract, and fractions did not show mortality in the animals at the limit dose of 2000mg/kg during the observation period. The outcome of the present study indicates that extract and different fraction shows potential anti-oxidant activity. Cladodes also extract significantly decreases elevated level of blood glucose...
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Posted by admin on May 31, 2021 in |
Objective: Stem Bark, Root, and fruit of Ziziphus oenoplia (Rhamnaceae) has been used in traditional health systems to treat diabetes and many more disorders in southern Asia as India, Sri Lanka, and Burma. However, the anti-oxidant and hypoglycemic potential of the fruit of this valuable plant is not scientifically validated till date. The aim of the present study is to evaluate in vitro free radical scavenging and hypoglycemic effect of ethanol extracts and solvent fractions of Ziziphus oenoplia fruit. Methods: The ethanol extracts and three solvent fractions of Ziziphus oenoplia fruit were evaluated at different concentration (20- 640 µg/ml) for free radical scavenging activity by using in-vitro DPPH method and different concentration (25-800 µg/ml) for hypo-glycemic potentials by using α – glucosidase and α-amylase enzymes (intestinal enzymes which slow the digestion of carbohydrates and delay glucose absorption) inhibitory activity. Results: The outcome of present study indicates that maximum anti-oxidant activity was produced by ethanol crude extract (87.66±1.54% Inhibition at 640 μg/mL) with IC50 value 2.72±0.62 followed by chloroform fraction...
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