Posted by admin on Jun 30, 2025 in |
This work aims to contribute to the phyto-therapeutic treatment of oral diseases that do not have a recognized management program, such as malaria, HIV-AIDS and tuberculosis. The aim is to qualitatively and quantitatively determine the chemical composition of Anacardium occidentale and Cocos nucifera, as well as the toxicity and sensitivity to their extracts of the microbial strains responsible for oral infections. Medicinal plant sellers in 03 markets of Porto-Novo use plant species to treat oral infections. C. nucifera (A) and A. occidentale (B) were the most frequently cited, used in decoction form. Qualitative phytochemical analysis using the standard method showed that C. nucifera roots contain tannins, steroids, mucilages, leuco-anthocyanins and alkaloids, while A. occidentale roots contain tannins, flavonoids, anthocyanins, leuco- anthocyanins, steroids, reducing compounds, coumarins and mucilages. The contents of total phenols (18.79±0.46 mgEAG/g(A)) and (20.05±1.01 mgEAG/g (B)), flavonoids (39.04±18.8 mgEQ/g) for extract B, condensed tannins (8.36±0.22 mgEC/g (A)) and (5.77±0.27mgEC/g(B)), justify the biological potential of these plants. Evaluation of the free radical scavenging activity of the decocts of...
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Posted by admin on Jun 30, 2025 in |
Objective: Knowledge about medicinal plants can contribute to modern therapeutics. Impatience walleriana [Family: Balsaminaceae] an ornamental, nutritive herb has relevance in African and Asian traditional medicines. Traditionally I. walleriana is used for anti-inflammatory, anti-dermatitis effects and is known to relieve skin irritation. It is applied on skin to treat bacterial and fungal infection. Acne is a common skin disorder mainly seen in adolescents. Treatment for acne revolves around anti-bacterial, anti-inflammatory and antioxidant effects of drugs. Anti-bacterial and antioxidant activities of I. walleriana have been reported previously reported. In the present study anti-acne potential of I. walleriana extract is evaluated against Propionibacterium acne and an attempt has been made to co-relate findings with traditional claims. Methods: Hydroalcoholic extract of aerial parts of I. walleriana is evaluated for effectiveness against P. acne by measuring zone of inhibition using well diffusion method and by finding MIC. Tetracycline is used as standard. Results: Results of Zone of Inhibition are promising with an average of 11mm, 08mm and 4mm in the duplicate readings obtained....
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Posted by admin on Jun 30, 2025 in |
Sickle cell disease (SCD) treatment and management remain a challenging puzzle, especially among developing Nations. We evaluated catechin’s sickling-suppressive properties using in-vitro and bioinformatics approaches in human sickle erythrocytes. Sickling was maximally induced (76%) using 2% sodium metabisulfite (SMS) at 3h. Addition of catechin prevented the sickling by SMS at 1mM (81.19%) and reversed the same at 1mM (84.63%), with IC50 values of 1.026µM and 1.103µM, respectively. Based on functional chemistry, catechin alters the functional groups of certain notable compounds within erythrocytes, favouring its anti-sickling effects, as indicated by the observed bends and shifts. From GC-MS and LC-MS analyses, it was observed that catechin treatment favours fatty acid alkyl monoesters (FAMEs) production with concomitant shutting down effects on selenocompound metabolism. Pathway enrichment and topology analyses revealed activation of fatty acid biosynthesis, linoleic acid metabolism and steroid hormone biosynthesis pathways upon catechin treatment. Thus, sickling-suppressive effects of catechin could potentially be associated with modulation of oxygenated and deoxygenated haemoglobin via alteration of human sickle erythrocytes’ functional chemistry and metabolic pathways...
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Posted by admin on Jun 30, 2025 in |
Dental caries, a widespread infection caused by cariogenic bacteria such as Streptococcus mutans, is traditionally managed in Nigeria using chewing sticks like Zanthoxylum zanthoxyloides and Nauclea latifolia. This study compares the anti-caries activity of these two plants and provides a scientific basis for their traditional use. The stems of Z. zanthoxyloides and N. latifolia were collected, identified, and subjected to powder microscopy, proximate analysis, phytochemical screening, and GC-MS analysis. The antimicrobial activity of the extracts was tested against Staphylococcus aureus, Streptococcus spp., and Candida albicans. Both plants exhibited significant antibacterial activity, with Z. zanthoxyloides showing effectiveness against S. aureus even at low concentrations, while N. latifolia was broadly effective against all tested organisms. Phytochemical screening revealed the presence of alkaloids, flavonoids, tannins, and steroids in both extracts, with GC-MS analysis identifying key bioactive compounds. Incorporating Z. zanthoxyloides into a plain toothpaste enhanced its antimicrobial activity, suggesting its potential in modern preventive dental care. These findings validate the traditional use of Z. zanthoxyloides and N. latifolia as effective oral hygiene...
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Posted by admin on Jun 30, 2025 in |
To perform phytochemical screening, estimate total phenolics and total flavonoid content of hydro alcoholic extract of dried leaves of Portulaca grandiflora Hook. The shade- dried leaves of P. grandiflora (100 g) were extracted exhaustively by Soxhlet with hydro- alcohol. The conc. hydro-alcoholic extract were further studied to quantitative determination, GC-MS and HPTLC to identify and characterize the chemical compound present in the crude extract. The phenolic content present in hydro-alcoholic leaf extract was found to be 1.57µg/ml (µg of Gallic acid/mg of extract) and flavonoid content was found to be 2.73µg/ml (µg of Rutin/mg of extract). Phytochemical analysis of hydroalcoholic extract showed presence of major classes of phytochemicals. High performance – thin layer chromatography results revealed the mobile phase of ethyl acetate: formic acid: glacial acetic acid: water (10:0.5:0.5:1.3), the fluorescent band under (254 nmand366 nm) at Rf 0.21 was identified, and the marker chemical rutin was measured. Gas chromatography – mass spectroscopy results showed the presence of 27 phytoconstituents including phenolic acids, ketones, flavonoids, carbohydrates, and hydrocarbons. The...
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