Posted by admin on Jun 1, 2014 in |
16 different bacteria were isolated from soil collected from and near Aurangabad city. All of the isolates were screened for L-asparaginase activity using modified M-9 medium. Spectro-photometric determination of L-asparaginase was done, and it was partially purified using ammonium sulphate precipitation. Out of the bacterial species screened only two were found to be producers of L-asparaginase. The Biochemical identification revealed both the species to be of the genus Bacillus. The crude extract showed a low enzyme activity as compared to partially purified extract. Both the isolates gave a maximum enzyme activity at pH 8 and a temperature of 40 °C. The genus Bacillus has become a promising candidate for the production of L-asparaginase from the other reported...
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Posted by admin on Jun 1, 2014 in |
This study was aimed to investigate the analgesic activity of the different fractions of Typha elephantina Roxb. Analgesic activity was evaluated for peripheral pharmacological actions using the acetic acid-induced writhing test in Swiss albino mice. The result of preliminary phytochemical screening reveals that different fractions of Typha elephantina Roxb. contains alkaloids, flavonoids, tannins, and carbohydrates. All fractions at the doses of 200 and 400 mg/kg b.w. they have produced significant (P<0.05) analgesic action in a dose-dependent manner in the tested model. In acetic acid-induced writhing test, petroleum ether, carbon tetrachloride and ethyl acetate fractions of roots (400 mg/kg) inhibited maximum 62.59%, 66.14%, and 69.29% writhing respectively, whereas the writhing inhibition of the standard drug Diclofenac-Na (25 mg/kg) was...
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Posted by admin on May 1, 2014 in |
Pharmacognostical parameters for the leaves including petiole of Kailaspati was studied with the aim of drawing the Pharmacognostical standards for this species. Macroscopical and microscopical characters of leaves and petiole, powder characteristics studies, fluorescence studies and thin layer chromatographic studies of the leaves powder included. The study also deals with the preliminary phytochemical screening of the leaves and petioles of Kailaspati with various extracts such as petroleum ether to water. Glandular, non-glandular trichomes and starch grains were identified. The qualitative analysis of some secondary metabolites, to ascertain medicinal claims of this widely used medicinal plant. The results showed that the moderate presence of oils, alkaloids, glycosides, flavonoids, ketosteroids, amino acids, phenols, and triterpenoids. The study includes pharmacognostical standardization of Kailaspati with petiole first...
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Posted by admin on May 1, 2014 in |
The ethanol extracts of stem bark of Synedrella nodiflora belonging to the family of Asteraceae was studied for hepatoprotective activity against Swiss Albino Rats with liver damage induced by carbon tetrachloride (CCl4). It was found that the ethanol extract of Synedrella nodiflora at a dose of 250 mg/kg body weight exhibited significant protective effect by lowering the serum level of alanine aminotransferase (ALT) or serum Glutamate Pyruvate Transferase (SGPT), and 100 mg/kg body weight exhibited significant protective effect by lowering both the serum levels of alanine aminotransferase (ALT) or serum Glutamate Pyruvate Transferase (SGPT), aspartate aminotransferase (AST) or serum glutamate oxaloacetate transaminase (SGOT). The highest hepato-protective activity was observed for ethanol extract of Synedrella nodiflora at a dose of 250 mg/kg body weight, and the reduction of serum level of ALT, AST and serum bilirubin were 60.918 ± 0.7936, 81.479 ± 2.85039 and 0.5205 ± 0.0249 respectively. Since, result of biochemical studies of blood sample of carbon tetrachloride treated rats showed significant increase in the level of serum enzyme...
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Posted by admin on May 1, 2014 in |
Plants have been used for centuries as a remedy for human diseases because they contain phytochemical components of therapeutic values. The whole plant of the Trigonella foenum, Zea mays and Oryza sativa were selected for in this study to find out the phytochemicals and antioxidant property. Phytochemicals were extracted using various solvents such as Hexane, Ethyl acetate, and ethanol. Screening of phytochemicals showed positive results for the presence of flavonoid, alkaloid, phenols, coumarins, steroids, and quinones. Free radical scavenging activity was determined using DPPH is a stable antioxidant. Ethyl acetate extract of Zea mays showed better antioxidant activity at a concentration of 100-150 µg followed by ethanol extract. An ethanol extract of Trigonella foenum revealed antioxidant activity at a concentration of 100-150 µg followed by ethyl acetate extract. These activities may be due to the presence of flavonoid, alkaloid, phenols, and steroids in plant extracts. Antioxidant activity was not observed in Oryza...
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