Posted by admin on Apr 30, 2026 in |
The present study involves the development and validation of simple, accurate, and precise analytical methods for the simultaneous estimation of Alprazolam and Sertraline Hydrochloride in combined pharmaceutical dosage forms. Two methods were developed: UV spectrophotometry and reverse-phase high-performance liquid chromatography (RP-HPLC). The UV spectrophotometric method utilized the simultaneous equation method based on absorbance measurements at 260 nm and 273 nm, the respective λmax values of Alprazolam and Sertraline HCl. Both drugs obeyed Beer’s Law in the concentration range of 5–50 μg/mL, with high correlation coefficients, indicating good linearity. The method showed satisfactory precision and accuracy, making it suitable for routine analysis. The RP-HPLC method was performed using a C18 column and a mobile phase composed of methanol, acetonitrile, and phosphate buffer (pH 4.5) in the ratio 50:15:35 v/v, at a flow rate of 1 mL/min. Detection was carried out at 239 nm, and the retention times were found to be approximately 3.0 minutes for Alprazolam and 6.4 minutes for Sertraline HCl. The method was validated according to ICH guidelines, and parameters such as specificity,...
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Posted by admin on Apr 30, 2026 in |
The antihelmintic activity of Causonis trifolia (Family: Vitaceae), Alstonia scholaris (Family: Apocynaceae), Trewia nudiflora (Family: Euphorbiaceae), Echinochloa crus-galli (Family: Poaceae), Cynodon dactylon (Family: Poaceae), Clerodendrum viscosum (Family: Lamiaceae) sometimes Verbenaceae, Aegle marmelos (Family: Rutaceae), Mentha australis (Australian mint) (Family: Lamiaceae) was studied against adult earthworms (Pheretima posthuma). The extract showed anthelmintic activity at the doses of 25 mg/ml, 50 mg/ml, and 100 mg/ml, although the reference standard used was albendazole (10 mg/ml). But the 100 mg/ml had the higher strong anthelmintic effect. Causonis trifolia, Alstonia scholaris, Trewia nudiflora, Echinochloa crus-galli, Cynodon dactylon, Clerodendrum viscosum, Aegle marmelos, and Australian mint caused paralysis and death of the earthworms at this concentration in about 88 min and 229min, 423min and 461min, 247min and 319min, 468min and 540 min, 440 min and 520 min, 61 min and 215 min, 270 min and 346 min, 328 min and 366 min, respectively. However, the extract of Clerodendrum viscosum at 100 mg/ml induced paralysis and death at about 61 min and 215 min,...
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Posted by admin on Apr 30, 2026 in |
Nowadays, intensive use of several forms of synthetic antibiotics to treat bacterial infections has resulted dramatically in emergence of resistant pathogenic microorganisms, often due to indiscriminate use of these substances. The present study was carried to evaluate the antibacterial and antifungal activities of different concentrations of methanol extracts of Rhodomenia palmata and Sargassum swartzii collected from Manappad, the Gulf of Mannar Biosphere Reserve, Tamil Nadu, India. The methanol extracts of selected two marine macro algae were screened against Bacillus subtilis, B. pumilus, Micrococcus luteus, Staphyloccous aureus, Klebsiella pneumoniae, Escherichia coli, Enterococcus faecalis, Streptococcus pyogenes, Proteus mirabilis, Proteus vulgaris, Shigella flexneri, Salmonella enterica, Vibrio chloerae and five yeast viz., Candida albicans, C. glabarata, C. tropicalis, C. krusei, C. parapsilosis, C. guilliermondii, Merozyma gulliermondii and Candida lusitaniae and nine mould fungi namely Aspergillus niger, A. flavus, A. fumigatus and A. clavatus four dermatophytes viz., Trichophyton rubrum and T. mentagrophytes strains. The antimicrobial activity was determined the extent of inhibitory zones using disc diffusion method, Minimum inhibitory Concentration (MIC), Minimum Bactericidal Concentration...
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Posted by admin on Apr 30, 2026 in |
Herbal candy is an innovative solid oral dosage form designed for localized soothing relief in the oral cavity and throat. Three formulations (F1, F2, F3) were prepared using fennel (Foeniculum vulgare), ginger (Zingiber officinale), and lemon (Citrus limon) in a sucrose base by the heating and molding method with varying herbal concentrations. All formulations were evaluated for organoleptic properties, weight variation, hardness, dissolution time, surface pH, moisture content, ash value, melting point, and stability over four weeks. Formulation F2 demonstrated the optimum profile: smooth texture, green color, aromatic odor, hardness 6.8 kg/cm², dissolution time 13 min, surface pH 4.5, weight 5.4±0.04 g, and complete stability at 25°C and 35°C for four weeks. The study confirms herbal candy as a safe, patient-friendly dosage form for mild throat discomfort, combining traditional herbal knowledge with modern pharmaceutical formulation...
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Posted by admin on Apr 30, 2026 in |
The present study evaluated the antiarthritic activity of the ethanol extract of Tephrosia purpurea (EETP) in experimental animal models. Acute oral toxicity studies revealed that EETP is safe up to 2000 mg/kg, with no mortality or observable toxic effects. Antiarthritic activity was assessed using parameters such as body weight, paw volume, mechanical withdrawal threshold, and thermal withdrawal latency in arthritic rats. EETP treatment showed dose-dependent improvement across all parameters. At 400 mg/kg, EETP significantly restored body weight, reduced paw edema, and improved both mechanical and thermal hyperalgesia, comparable to the standard drug Diclofenac. The findings suggest that EETP possesses significant anti-inflammatory and anti-nociceptive properties, supporting its potential as a therapeutic agent in the management of...
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