Posted by admin on Aug 31, 2026 in |
The increasing global use of Ayurvedic and herbal drugs is attributed to their therapeutic efficacy and reduced adverse effects when compared to conventional medicines. However, concerns regarding adulteration and lack of standardization pose challenges to their effectiveness. This study aims to formulate and evaluate the standardization parameters of an Ayurvedic formulation Polyherbal Digestive Churna through comprehensive analysis based on the guidelines of the World Health Organization (WHO) and the U.S. Food and Drug Administration (FDA). Polyherbal Digestive Churnas are commonly used in Ayurveda for treating various digestive disorders, including Adhmana, Sula, Gulma, and Agnimandya. The present paper reports the preparation and standardization of a polyherbal digestive churna which contain Mentha piperita L. (Peppermint leaves), Anethum graveolens (Dill fruits), Kaempferia galanga (Aromatic Ginger rhizomes), Cyperus rotundus (Java Grass rhizomes) and Palm candy. The preparation was carried out following the Indian Ayurvedic Pharmacopoeia to ensure optimal particle distribution and formulation consistency. A series of physicochemical, biological, and analytical tests were conducted, including flow properties, Carr’s Index, Hausner’s Ratio, angle of repose,...
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Posted by admin on Aug 31, 2026 in |
Talisadi Churna is a classical Ayurvedic polyherbal formulation traditionally used in cough, bronchitis, fever, dyspepsia, anaemia and cardiac disorders. The present study was carried out for pharmacognostical, physicochemical and phytochemical evaluation of standard laboratory-prepared and marketed formulations in accordance with WHO guidelines. Diagnostic microscopical features of each crude drug such as lignified fibres, stone cells, starch grains, oleo-resinous parenchyma, silicate crystals and epidermal characters were confirmed in all the formulations, ensuring its identity and authenticity. Phytochemical screening detected alkaloids, flavonoids, phenolics, tannins, carbohydrates, steroids and triterpenoids. Comparative TLC fingerprinting confirmed the presence of all crude drugs in prepared and marketed formulations. HPTLC quantification using piperine as a marker revealed highest content in P. nigrum (4.86%w/w), followed by P. longum (4.809% w/w). Among formulations, laboratory prepared contained 4.88% w/w, with comparatively lower amounts in marketed formulations. The findings provide a comprehensive standardization profile for Talisadi Churna, ensuring quality of both laboratory-prepared and marketed...
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Posted by admin on Aug 31, 2026 in |
The present study evaluated the phytochemical composition and antifungal activity of ethanolic extracts of five medicinal plants: Azadirachta indica (Neem), Ocimum sanctum (Tulsi), Allium sativum (Garlic), Curcuma longa (Turmeric), and Syzygium aromaticum (Clove). Preliminary phytochemical screening revealed the presence of alkaloids, flavonoids, tannins, glycosides, terpenoids, steroids, saponins, and phenolic compounds, with flavonoids and phenolics being the predominant constituents. Antifungal activity was assessed against Candida albicans and Aspergillus niger using the agar well diffusion method. Garlic extract exhibited the highest antifungal activity among the plant extracts, followed by clove and neem, while tulsi showed the lowest activity. Fluconazole demonstrated the greatest inhibition against both fungal strains. The findings support the traditional use of these medicinal plants and suggest that garlic and clove possess promising potential as natural antifungal agents for future therapeutic...
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Posted by admin on Aug 31, 2026 in |
Disorders related to female hormonal imbalance can profoundly and permanently affect quality of life. This study aimed to evaluate the estrogenic effects of an aqueous extract of Ricinodendron heudelotii almonds in ovariectomized rats. The seeds, harvested in Andé (Ivory Coast), were dried, ground, and then extracted with distilled water. A phytochemical screening was conducted to identify the main groups of secondary metabolites present in the extract. Acute toxicity was evaluated in six Swiss mice in accordance with OECD Guideline 423. Estrogenic activity was studied in ovariectomized Wistar rats using the OECD 440 uterine trophism bioassay. The animals were divided into four groups: control, ethinyl estradiol (0.02 mg/kg), 100 mg/kg of extract, and 300 mg/kg of extract. The treatments were administered orally daily for seven days. Body weight, as well as the relative weights of the uterus, cervix, and adrenal glands, were assessed. Vaginal smears were also collected to analyze cytological changes in the vaginal epithelium. Phytochemical analysis revealed the presence of sterols, polyterpenes, and alkaloids. No deaths or obvious...
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Posted by admin on Jul 31, 2026 in |
Curcumin, the principal polyphenolic constituent of Curcuma longa (turmeric), possesses potent anti-inflammatory activity. Despite remarkable pharmacological potential, clinical translation of curcumin is severely restricted by its drawbacks. The present study aimed to develop, optimize, and evaluate curcumin-loaded chitosan nanoparticles using Box-Behnken Design (BBD), and to compare their in-vitro antiinflammatory activity with free curcumin and the reference. Curcumin was extracted from dried Curcuma longa rhizomes and purified by silica gel column chromatography. An HPLC-UV analytical method was developed and fully validated per ICH Q2(R1) guidelines. The optimized formulation was characterized by dynamic light scattering (DLS), TEM, SEM, FTIR, DSC, and XRPD. In-vitro drug release, antiinflammatory activity and stability studies were conducted. Extracted curcumin showed 98.6% HPLC purity and confirmed identity by UV, FTIR, and melting point. BBD optimization yielded an optimal formulation with particle size 186.4 ± 7.8 nm, PDI 0.194 ± 0.018, zeta potential +28.6 ± 2.4 mV, and encapsulation efficiency 87.2 ± 1.6%. Solid-state characterization confirmed amorphous dispersion of curcumin within the chitosan matrix. Drug release followed 84.6%...
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