Posted by admin on Feb 28, 2025 in |
Kigelia pinnata Linn (Balam Kheera) Belongs to the family Bignoniace and commonly called as “Sausage tree” because of its hugs fruits. To study pharmacognosy and phytochemistry of kigelia pinnata and also studying Ayurveda, Siddha, Unani system of medicine. Kigelia Africana (lam) Benth. It is one of those tree species that have been heavily exploited for their medicinal, religious and cultural values. These species can reach 20 meters in height, sausage like in appearance with long cord-like talks. The species is native in Africa. Preliminary phytochemical screening performed on chloroform, petroleum ether, methanol, ethanol showed the presence of alkaloids, glycosides, flavonoids, steroids and tannins. The chemical constituent present in Kigelia pinnata is Naphthoquinone lapachol, pinnatal, isopinnatal, steroid is naphthoquinone, saponin, tannins, flavonoids. The collection of Kigelia pinnata leaves, fruits, stem, flowers etc. To study pharmacognostic characteristics of Kigelia pinnata leaves. The performance of the phytochemical screening of Kigelia pinnata leaves based on their physiochemical studies. To isolate pure phytoconstituent from extract by thin layer chromatography and elucidate structure of compound...
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Posted by admin on Feb 28, 2025 in |
Natural plant extracts have gained interest in cancer research due to their potential therapeutic properties. This study investigates the in-vitro cytotoxic effects and phytochemical composition of Mammea africana stem bark and Calophyllum inophyllum leaves methanol extracts against H460 lung cancer cells using the MTT assay, with 5-Fluorouracil (5FU) as a standard control. Phytochemical screening revealed that M. africana had high terpenoid content and moderate levels of flavonoids, tannins, and phenolics, while C. inophyllum had higher flavonoid and phenolic content, moderate saponins and terpenoids, and lower tannins and alkaloids. The extraction yields were 12.5% w/w for M. africana and 9.8% w/w for C. inophyllum. Cytotoxicity analysis demonstrated that M. africana exhibited strong cytotoxic effects, with an IC₅₀ value of 2.60 µg/mL, comparable to 5-Fluorouracil (IC₅₀ = 2.71 µg/mL), while C. inophyllum showed moderate cytotoxic activity (IC₅₀ = 30.00 µg/mL). The cytotoxic effects were dose-dependent, with M. africana achieving 97.47% cytotoxicity at 100 µg/mL, whereas C. inophyllum reached 85.4% at the same concentration. The findings suggest that the potent cytotoxic effects of Mammea africana could be attributed to its high terpenoid and tannin content, whereas the moderate activity of C. inophyllum may be linked...
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Posted by admin on Feb 28, 2025 in |
Cancer is a dangerous global disease and causes a heavy economic burden in low-income countries. Traditional medicinal plants play an important role in the research and development of cancer treatment drugs. Twenty-four traditional Vietnamese herbs were evaluated for their cytotoxic activities against nine cancer cell lines including HepG2, A549, MCF7, HT-29, HeLa, RD, LNCaP, HL-60 and NTERA-2. Cytotoxic activity was assessed following Monks’ protocol (1991) by measuring total cellular protein content using sulforhodamine B (SRB) staining. The 70% ethanol extract of eight medicinal herbs including Phyllanthus urinaria L. (TD.MD1), Solanum trilobatum L. (TD.MD2), Angelica sinensis (Oliv.) Diels (TD.MD3), Xanthium strumarium L. (TD.MD11), Scutellaria baicalensis Georgi (TD.MD15), Platycodon grandiflorus (Jacq.) A.D.C. (TD.MD17), Lycopodium clavatum L. (TD.MD20) and Ophiopogon japonicus (Thunb.) Ker Gawl. (TD.MD21) showed cytotoxic activity against HepG2, A549, MCF7, HT-29, HeLa, RD, LNCaP, HL-60 cell lines with IC50 values ranging from 18.60 to 93.84 µg/mL. The hexane, ethyl acetate, n-butanol, water extract residues of the 70% ethanol extracts demonstrated cytotoxic activities against NTERA-2 cell line with IC50 values of...
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Posted by admin on Feb 28, 2025 in |
This research demonstrates the use of inductively coupled plasma-mass spectrometry (ICP-MS) following microwave digestion in the estimation of the quantity of iodine in pharmaceutical samples and herbal formulations. The samples were dissolved in 4 mL of a 2% Tetra-methyl-ammonium-hydroxide solution, and iodine was used as a reference standard. Collectively, the findings show that the method was linear with calibration curves ranging from 1 ppb to 100 ppb. The recoveries of the examined samples fell between 98 and 115%. The precision of repeatability for iodine concentration ranged from 2.11% to 2.85% for intermediate precision. The present method is robust according to the percentage of RSD of robustness test under various modified conditions. For herbal formulations and pharmaceutical samples, validated methods were proved to be applicable, sensitive, accurate, and...
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Posted by admin on Jan 31, 2025 in |
Context: Epilepsy is a neurological disorder of brain in which the clusters of neurons, occasionally signal abnormally and cause strange emotions, sensations, and behavior, or sometimes muscle spasms, convulsions, and loss of consciousness. Lagenaria siceraria (Molina) Standley (LS), commonly known as “bottle gourd” (English), possesses several medicinal properties; little is known about its action as a nerve tonic. Objective: The purpose of this study was to characterize (or to study) the anti-epileptic potential of the bioactive fractions of methanolic extract of LS fruits through pharmacological screening. Materials and Methods: The experiment was conducted in specific animal models i.e., Pentylenetetrazole (PTZ)-induced convulsions and Maximal electroshock-induced seizures (MES) in Swiss albino mice to evaluate anti-epileptic effects. The effect of the fractions was also tested on motor coordination using the rota-rod apparatus. Results and Conclusions: Preliminary phytochemical screening of the methanolic extract of LS and its fractions showed a huge range of phytoactive compounds. Among all the fractions, chloroform and acetone fractions were found to have more number of such phytoactive compounds....
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