Posted by admin on Jan 31, 2020 in |
Realising the immense medicinal values of secondary metabolites in drug formulations, the present study was conducted on an important sec metabolite i.e., ellagic acid (tannin) in in-vivo (leaf) and in-vitro callus tissues of Woodfordia fruticosa a much used medicinally important plant. The present method provided a quick and easy approach for the detection and quantitation of biomarker ellagic acid. Qualitative analysis showed the presence of ellagic acid in both in-vivo and in-vitro (callus) samples of Woodfordia fruticosa. Analytical method high-performance thin-layer chromatography (HPTLC) was developed for the quantification of ellagic acid in in-vivo and in-vitro samples of the various solvent systems tried, the system containing toluene-ethyl acetate-formic acid-methanol (3:3:0.8:0.2 v/v) gave the best resolution of ellagic acid (Rf 0.65) from the other components of the sample extracts, and enabled their simultaneous quantification. Quantitative evaluation of the plate was performed in the remission/absorption mode at 280 nm by the use of a TLC Scanner III CAMAG. The amount of ellagic acid was estimated to be about 0.21% w/w for leaf...
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Posted by admin on Dec 31, 2019 in |
To analyze the efficacy of topical nepafenac, 0.1% eye drops in the treatment of diabetic macular edema (DME). Method: a prospective interventional study in 14 eyes having center involving DME were taken into consideration. They were administered topical nepafenac eye drops 0.1% thrice daily for 6 months. Their vision and foveal thickness were recorded systematically at the start of the treatment and then at 2nd, 4th, and 6th month follow up. Results: The mean baseline and final Log MAR visual acuity were 0.33 and 0.15, respectively and the mean baseline and final foveal thickness were 450 µm and 290 µm respectively. Conclusion: A statistically significant improvement was noticed in the visual acuity and foveal thickness after the administration of 0.1% of topical nepafenac eye...
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Posted by admin on Dec 31, 2019 in |
Plants and fruits have medicinal purposes, and they are being used long before the prehistoric period. Among the different varieties of citrus fruits, Citrus aurantifolia (Kagoji labu) is widespread in tropical and subtropical regions around the World. It is one of the major citrus fruits and widely consumed, but there is limited evidence about its health-promoting properties. In the present study, we evaluated the phytochemical and antioxidant properties of this citrus fruit since phytochemicals through anti-oxidant properties have played a significant role in human health protection and treatment of many diseases. Polyphenol, flavonoid, tannin and reducing sugar contents were found 4.68 ± 0.36 g GAE / 100 g extract, 615.38 ± 17.75 mg CE / 100 g extract, 12.5 ± 6.25 g TAE / 100 g extract and 51.69 ± 7.50 g GE / 100 g extract, respectively. Significant antioxidant activities of C. aurantifolia via 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and ferric reducing antioxidant power (FRAP) were detected. Earlier studies have reported that almost all species of citrus...
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Posted by admin on Dec 31, 2019 in |
The objective of this study was the extraction, recuperation, identification and bioactivity evaluation of the main bioactive compounds present in Chrysactinia mexicana A. Gray, polyphenolic compounds from aerial parts of Chrysactinia mexicana A. Gray were purified using a liquid chromatography column with ambulate XAD-16, first elution was made with distilled water, then ethanol was used to recover the phenolic fraction. These compounds were screened for antibacterial activity using agar diffusion method and antioxidant potential method was determined by ABTS•+, DPPH• and FRAP methods. Most bioactive compounds studied were effective against Klebsiella pneumoniae ATCC 13883, Escherichia coli ATCC 8739 and Salmonella enteric ATCC 7251, the compounds presented moderate inhibition against Staphylococcus aureus CDBB 1005. The polyphenolic profile was determined by HPLC/ESI/MS. The content of total hydrolyzable polyphenols (THP) after purification was 18.17 mgg -1, the content of total condensed total condensed polyphenols (TCP) determined after the extract purification was 18.25 mgg -1. The antioxidant activity of polyphenolic compounds of C. Mexicana (CMG) was determined by the IC50 of ABTS•+– scavenging...
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Posted by admin on Nov 30, 2019 in |
Salacia oblonga wall, a native shrub, also known as Saptrangi and Ponkoranti, belongs to the family Celastraceae, is distributed across the world. A large number of chemical constituents such as salacinol, kotalanol, neokotalanol, neosalacinol, and mangiferin are isolated from S. oblonga, which show various pharmacological activities. Salacia oblonga wall is being used in several herbal preparations for treating diabetes and obesity. It possesses anti-inflammatory, antihyperlipidemic, antiperoxidative, antimicrobial, antimutagenic, nephroprotective, and antimutagenic activities. Salacia oblanga has a long history of use as a treatment for diabetes in Ayurveda, traditional Indian medicine. Mugs made from Salacia wood are used by people with diabetes to drink water. In addition to treating diabetes, Salacia is used for treating gonorrhea, asthma, itchiness, joint pain (rheumatism), obesity, thirst, and menstrual problems. Salacia oblonga, an inhabitant of tropical regions, has been used in traditional Indian medicinal systems. Phytochemicals were extracted in methanol from the plant and analyzed for various anticancer activity. This research focuses on extraction, phytochemical investigation of Salacia, and the potential of Salacia oblonga wall in breast cancer by using in-vitro anti-cancer testing...
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