Posted by admin on Dec 31, 2019 in |
The objective of this study was the extraction, recuperation, identification and bioactivity evaluation of the main bioactive compounds present in Chrysactinia mexicana A. Gray, polyphenolic compounds from aerial parts of Chrysactinia mexicana A. Gray were purified using a liquid chromatography column with ambulate XAD-16, first elution was made with distilled water, then ethanol was used to recover the phenolic fraction. These compounds were screened for antibacterial activity using agar diffusion method and antioxidant potential method was determined by ABTS•+, DPPH• and FRAP methods. Most bioactive compounds studied were effective against Klebsiella pneumoniae ATCC 13883, Escherichia coli ATCC 8739 and Salmonella enteric ATCC 7251, the compounds presented moderate inhibition against Staphylococcus aureus CDBB 1005. The polyphenolic profile was determined by HPLC/ESI/MS. The content of total hydrolyzable polyphenols (THP) after purification was 18.17 mgg -1, the content of total condensed total condensed polyphenols (TCP) determined after the extract purification was 18.25 mgg -1. The antioxidant activity of polyphenolic compounds of C. Mexicana (CMG) was determined by the IC50 of ABTS•+– scavenging...
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Posted by admin on Nov 30, 2019 in |
Salacia oblonga wall, a native shrub, also known as Saptrangi and Ponkoranti, belongs to the family Celastraceae, is distributed across the world. A large number of chemical constituents such as salacinol, kotalanol, neokotalanol, neosalacinol, and mangiferin are isolated from S. oblonga, which show various pharmacological activities. Salacia oblonga wall is being used in several herbal preparations for treating diabetes and obesity. It possesses anti-inflammatory, antihyperlipidemic, antiperoxidative, antimicrobial, antimutagenic, nephroprotective, and antimutagenic activities. Salacia oblanga has a long history of use as a treatment for diabetes in Ayurveda, traditional Indian medicine. Mugs made from Salacia wood are used by people with diabetes to drink water. In addition to treating diabetes, Salacia is used for treating gonorrhea, asthma, itchiness, joint pain (rheumatism), obesity, thirst, and menstrual problems. Salacia oblonga, an inhabitant of tropical regions, has been used in traditional Indian medicinal systems. Phytochemicals were extracted in methanol from the plant and analyzed for various anticancer activity. This research focuses on extraction, phytochemical investigation of Salacia, and the potential of Salacia oblonga wall in breast cancer by using in-vitro anti-cancer testing...
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Posted by admin on Nov 30, 2019 in |
The study was designed to investigate the pharmacognostic characters and phytochemical profile of crude drugs obtained from powdered aerial parts of Phyllanthus maderaspatensis L. (Madras nelli). The macroscopic, microscopic, phytochemical screening, and HPTLC studies of powdered aerial parts of the plant were carried out. Powder microscopy of aerial part powder of Madras nelli showed an isocytic type of stomata and with the straight cell wall. Treatment of aerial parts powder of Madras nelli showed a positive test for starch, tannins, and lignins, whereas negative for oil globules and crystals. Loss on drying was 5%, Total Ash value, and Acid – insoluble Ash value was found to be 10% and 2%, respectively. Aqueous and Alcohol extractive values were found to be 11.80% and 9.80%, respectively. The phytochemical screening test revealed for the presence of carbohydrates and in ethanol and water extracts, phytosterols in ether extract, flavonoids, and tannins in ethanol extract and saponins in ethanol and water extract. HPTLC study of the alcoholic extract revealed five phytoconstituents at Rf -0.49,...
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Posted by admin on Nov 30, 2019 in |
Taverniera cuneifolia (Roth) Arn. licorice of India comprises similar phytochemical constituents majorly the Glycyrrhizic acid (GA) that of plant Glycyrrhiza glabra L. but has been least studied in terms of their commercial applications. GA from the root extracts of T. cuneifolia exhibits promising anti-inflammatory, anti-tumor, and anti-germ tube formation protection and other cytotoxic activities. To examine the same, present study experiments on methods for 1. Development of somatic embryo from root culture 2. To regenerate plants using root cultures. The experimental results showed the shoot initiation and increased rate of root growth, ranging from 65.4% to 89.3%, while plant regeneration from somatic embryos was 82.6% when cultures in ¼th strength Murashige and Skoog (MS) medium with 2% sucrose as a supplement. The study results in proliferate germination of seeds in respective media and suggests that T. cuneifolia could be used as a substitute of G. glabra in the practice of efficient mass production of plants from a single mother...
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Posted by admin on Nov 30, 2019 in |
Ayurveda an ancient system of medicines detailing a number of medicinal plants and their activities in humans or animals. The present research work aimed to develop an analytical procedure for the determination of catechins in the B. ciliata. It is famously known as a stone flower/stone breaker having biological activities like anti urolithiasis, antiviral, antidiabetic antitumor, and cardioprotective activity. The methanolic extract of the plant is isolated, and an analytical method is developed by using RP HPLC for the determination of catechins in the crude plant extract using a C18 column (200 × 4.6 mm, 5 µ) and detected at 241 nm. The method is validated for its system suitability, linearity, accuracy, precision, robustness, and sensitivity as per the ICH guidelines Q2 (R1) to meet the analytical procedure in academic and industrial...
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