POLYPHENOLIC PROFILE ANTIOXIDANT POTENTIAL AND ANTIMICROBIAL ACTIVITY OF CHRYSACTINIA MEXICANA A. GRAY (HIERBA DE SAN NICOLAS)

The objective of this study was the extraction, recuperation, identification and bioactivity evaluation of the main bioactive compounds present in Chrysactinia mexicana A. Gray, polyphenolic compounds from aerial parts of Chrysactinia mexicana A. Gray were purified using a liquid chromatography column with ambulate XAD-16, first elution was made with distilled water, then ethanol was used to recover the phenolic fraction. These compounds were screened for antibacterial activity using agar diffusion method and antioxidant potential method was determined by ABTS^•+, DPPH^• and FRAP methods. Most bioactive compounds studied were effective against Klebsiella pneumoniae ATCC 13883, Escherichia coli ATCC 8739 and Salmonella enteric ATCC 7251, the compounds presented moderate inhibition against Staphylococcus aureus CDBB 1005. The polyphenolic profile was determined by HPLC/ESI/MS. The content of total hydrolyzable polyphenols (THP) after purification was 18.17 mgg ^-1, the content of total condensed total condensed polyphenols (TCP) determined after the extract purification was 18.25 mgg ^-1. The antioxidant activity of polyphenolic compounds of C. Mexicana (CMG) was determined by the IC₅₀ of ABTS^•+– scavenging capacity (86.07 µgml ^-1). Anthocyanins, flavonoids and organic acids among which Petunidin 3, 5-O diglucoside, quercetin 3-O-glucoside and chicoric acid were identified in this plant material.