PHYTOCHEMICAL SCREENING, ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR THE DETERMINATION OF CATECHINS IN B. CILIATA BY RP-HPLCAbstract
Ayurveda an ancient system of medicines detailing a number of medicinal plants and their activities in humans or animals. The present research work aimed to develop an analytical procedure for the determination of catechins in the B. ciliata. It is famously known as a stone flower/stone breaker having biological activities like anti urolithiasis, antiviral, antidiabetic antitumor, and cardioprotective activity. The methanolic extract of the plant is isolated, and an analytical method is developed by using RP HPLC for the determination of catechins in the crude plant extract using a C18 column (200 × 4.6 mm, 5 µ) and detected at 241 nm. The method is validated for its system suitability, linearity, accuracy, precision, robustness, and sensitivity as per the ICH guidelines Q2 (R1) to meet the analytical procedure in academic and industrial usage
N. D. V. R. Saradhi *, N. M. Gopal, C. M. Chetty and K. Sushmitha
Santhiram College of Pharmacy, Nerawada, Kurnool, Andhra Pradesh, India.
30 October 2019
25 November 2019
27 November 2019
30 November 2019