PHYTOCHEMICAL SCREENING, ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR THE DETERMINATION OF CATECHINS IN B. CILIATA BY RP-HPLC
AbstractAyurveda an ancient system of medicines detailing a number of medicinal plants and their activities in humans or animals. The present research work aimed to develop an analytical procedure for the determination of catechins in the B. ciliata. It is famously known as a stone flower/stone breaker having biological activities like anti urolithiasis, antiviral, antidiabetic antitumor, and cardioprotective activity. The methanolic extract of the plant is isolated, and an analytical method is developed by using RP HPLC for the determination of catechins in the crude plant extract using a C18 column (200 × 4.6 mm, 5 µ) and detected at 241 nm. The method is validated for its system suitability, linearity, accuracy, precision, robustness, and sensitivity as per the ICH guidelines Q2 (R1) to meet the analytical procedure in academic and industrial usage
Article Information
3
360-364
423
313
English
IJP
N. D. V. R. Saradhi *, N. M. Gopal, C. M. Chetty and K. Sushmitha
Santhiram College of Pharmacy, Nerawada, Kurnool, Andhra Pradesh, India.
saradhi2u@gmail.com
30 October 2019
25 November 2019
27 November 2019
10.13040/IJPSR.0975-8232.IJP.6(11).360-64
30 November 2019
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