STANDARDIZATION OF AERIAL PARTS AND PHYTOCHEMICAL INVESTIGATION OF PLANT PHYLLANTHUS MADERASPATENSIS L. (MADRAS NELLI)Abstract
The study was designed to investigate the pharmacognostic characters and phytochemical profile of crude drugs obtained from powdered aerial parts of Phyllanthus maderaspatensis L. (Madras nelli). The macroscopic, microscopic, phytochemical screening, and HPTLC studies of powdered aerial parts of the plant were carried out. Powder microscopy of aerial part powder of Madras nelli showed an isocytic type of stomata and with the straight cell wall. Treatment of aerial parts powder of Madras nelli showed a positive test for starch, tannins, and lignins, whereas negative for oil globules and crystals. Loss on drying was 5%, Total Ash value, and Acid – insoluble Ash value was found to be 10% and 2%, respectively. Aqueous and Alcohol extractive values were found to be 11.80% and 9.80%, respectively. The phytochemical screening test revealed for the presence of carbohydrates and in ethanol and water extracts, phytosterols in ether extract, flavonoids, and tannins in ethanol extract and saponins in ethanol and water extract. HPTLC study of the alcoholic extract revealed five phytoconstituents at Rf -0.49, 0.60, 0.70, 0.72, 0.77.
R. R. Padhaya, S. Maharjan *, A. Khanal, T. Pokhrel and G. K. Burdipad
Central Institute of Science and Technology, New Baneshwor, Kathmandu, Nepal.
12 November 2019
26 November 2019
29 November 2019
30 November 2019