Posted by admin on Dec 1, 2014 in |
The leaf of Verbena officinalis was collected, air dried under the shed, powdered, soaked in and extracted with 80% methanol. The crude extract was tested for it’s in-vitro antibacterial activity against S. aureus and E. coli using agar disc diffusion method. Antibacterial assay was made using extract concentrations of 250 mg/ml, 500 mg/ml, and 1000 mg/ml. The extract showed a concentration-dependent zone of inhibition against the test bacteria. The strongest activity (largest zone of inhibition, 20.38 mm) was obtained at the concentration of 1000 mg/ml against S. aureus. The minimum inhibitory concentrations (MICs) of V. officinalis against S. aureus and E. coli were determined to be 0.019 mg/ml and 0.313 mg/ml respectively. Phytochemical analysis was also conducted on the powdered leaf of the plant. Seven secondary metabolites including chromophores, polyphenols, saponin, phytosterols, flavonoids, cardiac glycosides and tannins were detected from this medicinal plant. The observed antibacterial activity could be attributed to the presence of these secondary metabolites which have been associated with varying degrees of antibacterial activities. In conclusion,...
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Posted by admin on Nov 1, 2014 in |
Garlic is one of the earliest well known medicinal plants, which used to cure different disease in a human being. It used to fight infections, prevent cancer, antidiabetic, hepatoprotective, anthelmintic, anti- antioxidant, and wound healing. Based on the above important of garlic, this study was conducted to investigate the antibacterial activity of garlic extracts (ethanol, acetone and aqueous extracts) against bacterial strains. The antibacterial activity of garlic was tested against gram-positive Bacillus subtitles and gram-negative E. coli bacterial strains by using agar well diffusion method. The results reveal that garlic extract shows inhibitory activity against gram-positive bacteria Bacillus subtilis and gram-negative E. coli bacterial strains. The maximum zone of inhibitions observed against Bacillus subtilis with the combination (32 mm), aqueous (30 mm), ethanol (26 mm), and acetone (22 mm) extracts, respectively. Minimum inhibition zone was observed in all extracts against Gram-negative Bacteria (E. coli), i.e. combination (21 mm), aqueous (16 mm), ethanol (14 mm), and acetone (12 mm). The results showed that B. subtilus is more susceptible to garlic...
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Posted by admin on Nov 1, 2014 in |
Infertility is one of the most extremely tragic all over the world. Despite recent advances in the treatment of male infertility, the problem has not been satisfactorily tackled. The male infertility is mainly due to an inadequate number of spermatozoa in the semen, the failure of the spermatozoa to move with sufficient vigor towards their goal. Herbal medicines have been broadly used in developed countries hence they are natural and comparatively safe. They contain plant materials as their pharmacologically active components. The aim of the study is to evaluate the acute and sub-acute toxicity of the spermatogenic Siddha drug Magizhampattai Chooranam (MPC). For acute studies, different doses of MPC were administered orally to rats once daily for one week. For sub-acute studies, different doses of IC were administered orally to rats once daily for 28 days in various doses at 250, 500, 1000 mg/kg of body weight. Detailed hematological, biochemical, necropsy and Histopathological evaluation of organs were performed for all animals. Histopathological analysis revealed that Spleen, Testes, Pancreas, Lung,...
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Posted by admin on Nov 1, 2014 in |
Desmostachya bipinnata (L.) Stapf [Gramineae (Poaceae)] is used in Indian traditional medicines system for treatment of various diseases such as asthma, kidney stone, diarrhea, wound healing, etc. These traditional claims require authentication by performing animal testing. This study aims to confirm the antidiarrheal activity of D. bipinnata in Wistar rats. Underground parts of D. bipinnata was extracted in alcohol, water, a hydroalcoholic solution, chloroform, and ethyl acetate. Acute toxicity test was performed in albino mice and antidiarrheal activity was estimated in Wistar rats. Castor oil and magnesium sulfate-induced diarrhea in Wistar rats were treated with single oral administration of prepared D. bipinnata extracts (500 mg/kg) and antidiarrheal drug loperamide (3 mg/kg). Diarrhoeal droppings were observed for 6 h in all treated animals. One way ANOVA followed by Dunnet’s ‘t’ test was used to identify significant differences among obtained results. Extracts of D. bipinnata showed neither mortality nor any toxic effect in albino mice up to the dose of 5 g/kg in 48 h to 14 days. The alcohol extract of...
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Posted by admin on Nov 1, 2014 in |
The inhibitory effects of ethyl acetate, aqueous and ethanolic extracts of Talinum triangulare were well observed in the brain homogenate of Swiss albino rats against sodium nitroprusside induced-peroxidation by thiobarbituric acid reactive species (TBARS) in-vitro. All the extracts showed significant inhibitory abilities against sodium-nitroprusside (SNP) induced lipid peroxidation in the brain homogenate when incubated with 60mM sodium nitroprusside. In the current work, aqueous extract of Talinum triangulare seems to possess higher antioxidant contents with the fact that it shows the highest inhibition potential against SNP-induced peroxidation in the brain tissue homogenate from the results, thereby characterize the ability to protect the brain cell and as well able to prevent nitrosative stress induction at considerable measure in the...
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