Posted by admin on Feb 29, 2020 in |
Information from ethnomedicine suggests that Diospyros mespiliformis possesses antiulcer properties. Thus, the purpose of this study was to evaluate its gastroprotective activity. The gastroprotective activity of the leaves aqueous extract of D. mespiliformis was evaluated by three experimental models of gastric ulcers in mice, namely: HCl/ethanol, HCl/ethanol with indomethacin pre-treatment and indomethacin (p. o). Ulcerated surface, ulcer index, and mucus mass were recorded. A qualitative phytochemical analysis of the extract was carried out. The administration of D. mespiliformis extract prevented the formation of gastric lesions against the necrotizing agents. For HCl/ethanol induction, the extract at 100, 200, and 400 mg/kg dose-dependently inhibited ulcer formation of 28.36, 29.19 and 35.82%, respectively. Indomethacin pre-treatment reduced the preventive effect of the extract to 19.69 and 28.24% at 200 and 400 mg/kg, respectively. As for indomethacin induction, the extract at 200 mg/kg showed the most important ulcer inhibition (88.13%). For all three induction models, a significant increase in mucus secretion between 44.75 and 121.34% was observed. D. mespiliformis could prevent the formation of...
Read More
Posted by admin on Jan 31, 2020 in |
The objective of the present study is to develop the pharmacognostic and phytochemical profiles of Ruellia tuberosa L. leaf and root. The present study considers the detail investigation of macroscopy, microscopy, preliminary qualitative phytochemical analysis, physicochemical evaluations, and chromatography profiling. The microscopical investigation was adopted to identify the cellular powder characters of leaves and roots, including diacytic stomata, few sessile glandular trichomes, profuse oil globules, exfoliating cork, few small schizogenous oil cavities, cystolith, etc. Physicochemical parameters reveal the data of quality, purity while phytochemical screening reflects the presence of various secondary metabolites. The chromatographic fingerprint data represent the authenticity of plant sample. Data obtained from botanical and chemical screening in combination may be considered as standard for identification and authentication of leaves and roots and may be helpful in developing pharmacopoeial...
Read More
Posted by admin on Jan 31, 2020 in |
The objective of the study was to formulate and evaluate the antioxidant activity and the wound healing effect of the Punica granatum peel extracts formulation. Tannins, alkaloids, flavonoids, proteins, and phenols were present in the ethanolic extract, which is further formulated in a gel formulation. Seven herbal gel formulations were prepared using 1.5% of the gelling agent’s carbopol 940 (F1-F7). Formulations were evaluated for physical appearance, viscosity, extrudability, pH, and spreadability. The antioxidant study was evaluated in-vitro, using 2, 2-diphenylpicrylhydrazyl (DPPH), and reducing power assays. It was found that all the gel formulation has good power to inhibit DPPH and good reducing power. The antimicrobial properties were evaluated by agar well diffusion method. The result indicated that F5 and F7 exhibited good antimicrobial properties. The highest potential was observed in the F7 against E. coli and B. subtilis. Wound healing was studied using excision wounds on rat models. Treatment of wound with a gel containing 2% and 10% (w/w) ethanolic extract exhibited better-wound healing activity than the positive control....
Read More
Posted by admin on Jan 31, 2020 in |
Realising the immense medicinal values of secondary metabolites in drug formulations, the present study was conducted on an important sec metabolite i.e., ellagic acid (tannin) in in-vivo (leaf) and in-vitro callus tissues of Woodfordia fruticosa a much used medicinally important plant. The present method provided a quick and easy approach for the detection and quantitation of biomarker ellagic acid. Qualitative analysis showed the presence of ellagic acid in both in-vivo and in-vitro (callus) samples of Woodfordia fruticosa. Analytical method high-performance thin-layer chromatography (HPTLC) was developed for the quantification of ellagic acid in in-vivo and in-vitro samples of the various solvent systems tried, the system containing toluene-ethyl acetate-formic acid-methanol (3:3:0.8:0.2 v/v) gave the best resolution of ellagic acid (Rf 0.65) from the other components of the sample extracts, and enabled their simultaneous quantification. Quantitative evaluation of the plate was performed in the remission/absorption mode at 280 nm by the use of a TLC Scanner III CAMAG. The amount of ellagic acid was estimated to be about 0.21% w/w for leaf...
Read More
Posted by admin on Dec 31, 2019 in |
To analyze the efficacy of topical nepafenac, 0.1% eye drops in the treatment of diabetic macular edema (DME). Method: a prospective interventional study in 14 eyes having center involving DME were taken into consideration. They were administered topical nepafenac eye drops 0.1% thrice daily for 6 months. Their vision and foveal thickness were recorded systematically at the start of the treatment and then at 2nd, 4th, and 6th month follow up. Results: The mean baseline and final Log MAR visual acuity were 0.33 and 0.15, respectively and the mean baseline and final foveal thickness were 450 µm and 290 µm respectively. Conclusion: A statistically significant improvement was noticed in the visual acuity and foveal thickness after the administration of 0.1% of topical nepafenac eye...
Read More