IN-VITRO ANTIOXIDANT POTENTIAL, TOTAL PHENOLIC AND FLAVONOID CONTENT OF LAMIUM AMPLEXICAULE LEAF EXTRACT IN AQUA-METHANOL AND AQUA-ACETONEAbstract
Lamium amplexicaule L. is a genus of the family Lamiaceae. This plant species has been used widely in traditional medicines to treat many disorders. Phytochemical screening (qualitative and quantitative), antioxidant capacity, total phenol, and total flavonoid content were analyzed in a crude extract of leaves. The extract was prepared in aqua-methanol and aqua acetone in 30:70. Antioxidant capacity was analyzed in both extracts through (2,2-diphenyl-1picrylhydrazyl (DPPH) Free Radical Scavenging Assay, FRAP (Ferric Ion Reducing Antioxidant Potential), FCA (Fe2+ Ion Chelating Activity), Phosphomolybdenum assay (TAA), Total Phenol Content (TPC) and Total Flavonoid Content (TFC). Both extracts showed the presence of tannins, alkaloids, flavonoids, proteins, phenols, saponins, terpenoids, carbohydrates, quinones, oils, and resins. The result for antioxidant assay (DPPH, FRAP, FCA and TAA) was comparatively higher in aqua-methanol than in aqua-acetone. TFC values were higher in aqua-acetone, while TPC in aqua-methanol extract. Thus, the plant species can be used as a potent source of ayurvedic medicine.
Gayatri Thakurathi, P. B. Rao and Kamlesh Kumar Bhakuni ⃰
Department of Botany, L. S. M Government Postgraduate College, Soban Singh Jeena University, Almora, Uttarakhand, India.
01 August 2022
10 December 2022
12 December 2022
31 December 2022