Posted by admin on May 31, 2024 in |
In this study, Pleurotus ostreatus polysaccharide (POP) is isolated, refined, structurally analysed, and its anticancer properties are investigated. Polysaccharide yield was 6.27%, purified via DEAE-52 and Sephadex S-300 chromatography, with a molecular weight of 154649.8 Da. Structural analysis through FT-IR, HPLC, GC-MS, and NMR revealed POP as a homopolysaccharide composed of D-glucose units, featuring (1→6)-α-D-Glcp backbone with O-6 branches and T-α-D-Glcp terminations. These findings contribute to understanding the biological potential of POP. POP’s antitumor activity study showed that, with an IC50 of 121.801 µg/mL, it was highly cytotoxic to EAC cell types, supported by LDH release analysis. POP inhibited cell migration, invasion, and colony formation, indicating its potential as an anti-cancer agent. Analysis using flow cytometry showed that POP induced apoptosis, which raised the expression of Bax and Caspase-9 while decreasing the expression of Bcl-2. DNA fragmentation assay showed characteristic laddering pattern, confirming apoptosis-mediated DNA degradation. Furthermore, alterations in the expression of the proteins p53, Cyclin D, and Cdk4 were linked with POP-induced cell cycle arrest in the G0/G1...
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Posted by admin on May 31, 2024 in |
Irvingia gabonensis is a plant popularly used for both medicinal and dietary purposes. The planthave been used to treat various diseases like diabetes, inflammation, alopecia, fever and ulcers. The study was aimed at evaluating some pharmacognostic fingerprints and molecular DNA barcoding of the stembark. The pharmacognostic fingerprint analysis of the stembark was carried out using standard procedures while the molecular DNA barcoding was done using automated DNA extraction and barcoding by the polymerase chain reaction machine. The organoleptic evaluation showed that the greyish brown stembark of I. gabonensis has a strong bitter taste, acrid odour and slightly coarse to the touch. Chemomicroscopic evaluations reveal the presence of cellulose, suberin, lignin, mucilage, prismatic calcium oxalate crystals and tannins. The transverse section of the stembark showed bicollateral vascular bundle, beaded hexagonal shaped cork cells, fibre sclereids with wide cell lumen, non-striated eccentric oval shaped aggregate starch grains, and wide pith as observed under the scanning electron microscope. The moisture content was 5.13 ± 0.11 w/w %, total ash was 18.58 ±...
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Posted by admin on May 31, 2024 in |
The present work focused on preparing ethanolic extract of Nymphae nouchali flower and evaluating its anti-diabetic potential in rats. The extraction of flowers was carried out using ethanol and the extraction yield was found to be 18.6% w/w. The result of the total phenolic content of the extract examined using Folin-Ciocalteu method revealed total phenolic content of ethanolic extract of Nymphae nouchali was 41.26 ± 2.13 GAE mg/g. The antidiabetic action of the extract was evaluated in terms of the effect of extract on body weight and serum glucose level. It was found that alloxan administration reduced the body weight of animals significantly in comparison with the normal control animals. The results also revealed that the serum glucose levels were highly elevated by the administration of alloxan in all the groups by day 7. In the treatment groups III to V, the glucose levels dropped in comparison to group II and by day 21, the levels reached almost the level of control group in group III and V. This...
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Posted by admin on Apr 30, 2024 in |
Since time immemorial, natural products have been the backbone of traditional system of healing throughout the globe and have also been an integral part of history and culture. Hence Baphia pubescence has been used ethnomedicinally to treat various diseases. The leaf or leaf juice is applied against parasitic skin diseases. A leaf infusion is drunk to cure enteritis and other gastrointestinal problems. The leaves and bark are considered haemostatic and anti-inflammatory and are used for healing sores and wounds. The leaves infusion is used to treat malarial infections as well as other microbial infections. As a result, it is pertinent to study the toxicity of the plant. The toxicological study both acute and chronic were evaluated according to standard methods. Acute andChronic toxicity studies of B. pubescens indicated that the plant is relatively safe at the therapeutic dose. The toxicity of the plant was shown to be dose dependent. The kidney and liver enzymes increased with increasing doses whereas the histopathological damage after long administration on these organs was...
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Posted by admin on Feb 29, 2024 in |
Fast dissolving or mouth disintegrating tablets are oral delivery systems intended for a rapid breakdown of the dosage form in the mouth or oral cavity, in contrast to other conventional dose forms. Labetalol, an alpha-1-adrenergic antagonist of the third generation, has vasodilatory and antihypertensive properties. It is used to treat hypertension and angina. Using novel co-processed super disintegrants and physical combinations combining croscarmellose and crospovidone in 1:1, 1:2, 1:3, 2:1, and 3:1 ratios, this study attempts to produce and evaluate labetalol fast dissolving tablets. The direct compression method was used to make the tablets. The tablets work instantly since they dissolve quickly when used orally. Like liquid dosage forms, orally disintegrating tablets are easier to take and offer greater convenience. Important excipients called disintegrants are added to solid dosage forms in order to break or disintegrate tablets in aqueous media. The disintegrants enhance the formulation’s surface area, or tablet’s surface area, and counteract the cohesive forces that hold the particles...
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