Posted by admin on Mar 1, 2014 in |
Objective: Phytochemical and pharmacological investigation on the leaves of Mimusops elengi Linn. for wound healing and in-vivo antioxidant activity in albino mice. Material and Methods: The methanolic extract of leaves of Mimusops elengi was studied for its wound healing effect on excision wound model in mice and in-vivo antioxidant activity at the dose level using a biochemical estimation of blood serum in mice. Result: Preliminary phytochemical analysis revealed the presence of saponin, carbohydrate, glycosides, flavonoids, tannin and phenolic compounds. Mimusops elengi methanolic extract (MEME) was examined for wound healing activity in the form of ointment in excision wound model in mice. The extract ointment showed a considerable response in the above model as compared to standard drug Betadine ointment regarding wound contracting ability, wound closure time. Histological analysis was also consistent with the proposal that Mimusops elengi leaf extract exhibits significant wound healing effect. As antioxidant action has been reported to play a crucial role in the hepatoprotection in in-vivo model of antioxidant activity. It has been found that...
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Posted by admin on Mar 1, 2014 in |
Pharmaceutical preparations having high water content face the problem of microbial spoilage which affects consumer safety. Control on such problem is generally done by the addition of a specific preservative to these pharmaceutical preparations, but sometimes some physical changes and clinical hazards are observed even after the addition of preservatives. The present study aimed to evaluate and compare the effectiveness of preservatives in market samples of antacids, cough syrups, and ophthalmic solutions through a microbial challenge test. The samples of antacids, cough syrups, and ophthalmic solutions were challenged with 3 bacterial, and 2 fungal strains and results were periodically (0, 7, 14 and 28 day) investigated. The number of surviving microorganisms were determined using standard microbiological dilution pour-plate technique. More than 1 log reduction of microbial counts were observed in all samples at 7 day. Moreover, the log reduction in microbial counts was significantly increased up to 28 day. Results showed that preservatives Sorbitol, Sodium Citrate, and Benzalkonium Chloride present in antacids, cough syrups, and ophthalmic solutions respectively were...
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Posted by admin on Mar 1, 2014 in |
Rhizome of Kaempferia galanga (Family: Zingiberaceae) has been investigated for isolation of secondary metabolites and evaluation of bioactivities. Two cinnamic acid derivatives, Ethyl p-methoxycinnamate (KG-2) and 4-methoxy cinnamic acid (KG-9) have been isolated from the chloroform fraction of the methanolic extract of the rhizome of K. galanga using chromatographic analysis. Structures of these compounds were elucidated by extensive spectroscopic analysis and by comparing the data with the published one. In the carrageenan-induced paw edema test, at a dose of 400 mg/kg the methanolic extract, chloroform and n-hexane fraction showed a significant (p<0.05) inhibition of paw edema with 55.08, 48.85 and 71.88 % inhibition after a 2nd hour of the study period. In acetic- acid-induced writhing test, the chloroform and n-hexane extract at a dose of 400 mg/kg showed a significant (p<0.001) reduction in the number of writhes with38.19 and 62.31% writhing inhibition respectively. In hypoglycemic evaluation study by oral glucose tolerance test, all of the extracts at 200 mg/kg body weight inhibited the rise in blood glucose level. After...
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Posted by admin on Mar 1, 2014 in |
Equisetum arvense has a great medicinal value for its wound and burn healing properties. Traditionally, the plant is used by local people and Ayurvedic physicians mainly for its burn healing properties. The methanolic extract of Equisetum arvense was obtained by extraction. The present study focuses on the analysis of the methanol extract of Equisetum arvense by Gas Chromatography-Mass Spectrometry. The phytocomponents of the methanol extract of Equisetum arvense were investigated by using Gas Chromatography-Mass spectrometry, while the mass spectra of the compounds found in the extract were matched with the National Institute of Standards and Technology Library. The study revealed the presence of six...
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Posted by admin on Mar 1, 2014 in |
Inflammation is a body defence reaction to prevent the spread of injurious agent and to remove the necrosed cells and tissues. Inflammatory abnormalities are a large group of disorders which underlie a vast variety of human diseases. During treatment of inflammatory diseases, many conventional therapies (non-steroidal anti-inflammatory drugs) used to relief pain and inflammation. Continuous use of the intended drugs is frequently associated with serious side effects, whereas plants still hold their unique place, by way of having no side effects. Therefore, a systematic approach should be made to find out the efficacy of plants against inflammation to exploit them as herbal anti-inflammatory agents with a better safety profile. Luffa acutangula Linn. (Cucurbitaceae) has many therapeutic uses mentioned in Ayurveda and therefore we aimed to study its anti-inflammatory...
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