EVALUATION OF IN VITRO ANTIOXIDANT AND ANTI-PROLIFERATIVE EFFECT OF DEFATTED EXTRACT OF COCOS NUCIFERA KERNEL ON HUMAN BREAST CANCER CELL LINEAbstract
The present study was designed to evaluate the in vitro antioxidant effects and potential benefits of using coconut kernel extract in preventing the proliferation of human breast cancer cell line, MCF-7. Defatted extract from coconut kernel (CKf) was evaluated for its radical scavenging, prevention of human LDL oxidation, cytotoxic, apoptotic and nuclear effects on MCF-7 cells. Intracellular production of reactive oxygen species (ROS) and superoxide radical was studied. It was showed that CKf significantly scavenged free radicals and prevented LDL oxidation. MCF-7 cell viability was found to be decreased in a dose-dependent manner following treatment with CKf. AO-EB and DAPI staining confirmed that treatment with CKf, induced nuclear damage and apoptosis as distinctly visualized in cells. Our observations suggest that nuclear/cellular damage and poor mitochondrial membrane potential (MMP) appears to be an indirect consequence of the excessive generation of reactive oxygen species (ROS) and superoxide radicals.
R. Dhanyakrishnan, M. C. Sunitha, B. Prakashkumar, S. Sandya and K. G. Nevin*
School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala, India.
21 October 2017
14 November 2017
18 November 2017
01 March 2018