Posted by admin on Jun 1, 2014 in |
Kidney stone is a painful disease which is a major problem in all regions of Pakistan as this country is located in the so-called stone belt. Drastic changes in dietary habits including the use of soft drinks are increasing the incidence of kidney stone disease. To investigate this phenomenon, calcium oxalate monohydrate crystals were induced in artificial urine by mixing calcium chloride and sodium oxalate in-vitro and different types of soft drinks were analyzed for activity against calcium oxalate monohydrate type’s kidney stone. Most of them showed effects on calcium oxalate monohydrate to calcium oxalate dihydrate crystals under DIC microscopy. This study showed that slices had the most promising effect on calcium oxalate monohydrate crystals than Mirinda and Mountain dew. 7up and Pepsi did not show a change in morphology of the crystals of calcium oxalate...
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Posted by admin on Jun 1, 2014 in |
Acacia caesia L. (family Mimosaceae) is a well-known shrub in Southeast Asia, where the natives use it for a wide range of purposes. The Mizo tribes of India use the stem bark for the treatment of gastrointestinal infections. The present study is an attempt to elucidate structural changes on the tegument of chicken tapeworm Raillietina tetragona Molin. upon treatment with the methanol extract of A. caesia stem bark. R. tetragonal recovered from chicken intestines were treated with 20 mg/mL of the plant extract at 37 °C. The tapeworms were fixed in neutral-buffered formaldehyde at 4 °C, and then post-fixed in 1% osmium tetraoxide. They were completely dehydrated using acetone. After treating with tetramethylsilane and drying under room temperature, they were coated with gold. Then they were finally observed under a scanning electron microscope to observe the tegument. Tapeworm showed severe damage to the scolex. Suckers were obliterated. The tegument was distorted and shrunk. The fine microtriches were completely removed. A. caesia stem bark extract is, therefore, a potent anthelmintic...
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Posted by admin on Jun 1, 2014 in |
Calcium oxalate crystals have a definite biological function in medicinal plants and act as a good diagnostic tool for the identification and detection of adulterants in crude drugs. The occurrence of calcium oxalate crystals was evident in Moringa pterygesperma, Mentha arvensis, Cissus quadrangularis, Murraya koenigii and Amaranthus gangiticus leaves. Rosette, druse, raphides, acicular and prismatic crystals were recorded. The presence of calcium oxalate crystals was confirmed; further Cissus quadrangularis leaves had both druse and raphides belonging to type-II. Druse in Amaranthus gangiticus was the largest, measuring about 12.98-18.82 µm in length, 9.74-14.28 µm in breadth in the light microscope and 22.1 µm Scanning Electron microscopy. Mentha arvensis leaves showed acicular crystals as well as druse in the Scanning Electron Microscope...
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Posted by admin on Jun 1, 2014 in |
16 different bacteria were isolated from soil collected from and near Aurangabad city. All of the isolates were screened for L-asparaginase activity using modified M-9 medium. Spectro-photometric determination of L-asparaginase was done, and it was partially purified using ammonium sulphate precipitation. Out of the bacterial species screened only two were found to be producers of L-asparaginase. The Biochemical identification revealed both the species to be of the genus Bacillus. The crude extract showed a low enzyme activity as compared to partially purified extract. Both the isolates gave a maximum enzyme activity at pH 8 and a temperature of 40 °C. The genus Bacillus has become a promising candidate for the production of L-asparaginase from the other reported...
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Posted by admin on Jun 1, 2014 in |
This study was aimed to investigate the analgesic activity of the different fractions of Typha elephantina Roxb. Analgesic activity was evaluated for peripheral pharmacological actions using the acetic acid-induced writhing test in Swiss albino mice. The result of preliminary phytochemical screening reveals that different fractions of Typha elephantina Roxb. contains alkaloids, flavonoids, tannins, and carbohydrates. All fractions at the doses of 200 and 400 mg/kg b.w. they have produced significant (P<0.05) analgesic action in a dose-dependent manner in the tested model. In acetic acid-induced writhing test, petroleum ether, carbon tetrachloride and ethyl acetate fractions of roots (400 mg/kg) inhibited maximum 62.59%, 66.14%, and 69.29% writhing respectively, whereas the writhing inhibition of the standard drug Diclofenac-Na (25 mg/kg) was...
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