Posted by admin on Feb 5, 2018 in |
Dregea volubilis (L.f.) Benth. Ex Hook. f. is a large twinning shrub belonging to the family Asclepiadaceae. The leaf is amphistomatic with tetracytic, anomocytic, paracytic stomata. The sides of the leaf in the surface are straight to curved, surface striated on either side. The mesophyll is interspersed with sphaerocrystalliferous idioblasts. Midvein consists of a single arcuate vascular bundle, bicollateral, conjoint, endarch. In T. S. petiole is oval, adaxially grooved at center and with two lateral adaxial ridges, consisting of a large arc shaped vascular bundle at the centre with a 2 small spherical vascular bundles in adaxial...
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Posted by admin on Feb 5, 2018 in |
Antigonon leptopus Hook & Arn (family Polygonaceae), also called: Chain of love, Queen’sWreath, and Mexican creeper is a fast-growing vine with stems reaching up to 20 feet long. It has heart-shaped, green leaves climbing by tendrils which wrap around many types of supports. It is native to Mexico and commonly found in tropical Asia, Africa, the Caribbean, and the Americas. It possesses anticoagulant activity, analgesic, anti-thrombin, anti-inflammatory, anti-diabetic and antidepressant activities. Aerial parts of the vine have been used as hepatoprotective and for spleen disorders. During the present study, LC/MS experiment was conducted on the methanolic extract of the aerial parts of the vine. Results revealed the identification of total fifty compounds; flavonoids, phenolics, anthocyanins, and glycosides. Compounds were characterized by their retention times, mass spectra with comparison to online mass bank database. From the results, Antigonon leptopus can be incorporated in pharmaceutical supplements used to aid free radical-mediated disease; cancer, diabetes, inflammation and liver...
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Posted by admin on Jan 11, 2018 in |
Thevetia Peruviana (or Cascabela thevetia) belongs to the family Apocynaceae and commonly called as Yellow Oleander. The leaves of this significant medicinal plant were subjected to antibacterial investigation and HPLC analysis. Hexane, acetone, and methanol were used as organic solvents. Four different concentrations (200 mg/ml, 100 mg/ml, 50 mg/ml and 25 mg/ml) of each extract i.e. hexane, acetone and methanol were prepared for antibacterial activity by dissolving them in 100% DMSO. The leaf extracts were subjected to test the antibacterial activity by agar well diffusion method. The methanol extract of leaves showed significant antibacterial activity against Pseudomonas aeruginosa and poor activity against Micrococcus luteus and Shigella flexneri. Acetone extract showed moderate activity against the tested micro-organisms viz. Pseudomonas aeruginosa, Micrococcus luteus and Shigella flexneri. Hexane extract showed moderate activity against Micrococcus luteus and poor activity against Pseudomonas aeruginosa and Shigella flexneri at different concentrations. HPLC analysis of methanol leaves extract was performed which showed that plant leaf contains different types of phytosterols. Thevetia peruviana leaves a promising possibility of...
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Posted by admin on Jan 11, 2018 in |
In the present study, an attempt was made to formulate ethosomal gel containing leaf extract of Mangifera indica, and was evaluated for antibacterial activity against gram-positive and gram-negative organisms. Different formulations of ethosomes using lecithin, cholesterol, and ethanol were prepared using Mangifera indica herbal leaf extracts. Carbopol 940 was used to prepare ethosomal gel. The entrapment efficiency of ethosomes was 65.1% – 96.54% and the average vesicle size was 920 nm. Three formulations of different doses (100, 200 and 300 mg) were selected based on entrapment efficiency and drug release. Prepared gels were then evaluated for physicochemical characteristics and drug content. The antibacterial activity was done using agar well diffusion method for the zone of inhibition. The pH of the gel formulations was found to be in the range of 5.4 – 6.2 and viscosities between 2250 – 2399 centipoises. The drug content of gels ranged between 74.67 – 82.31%. The zone of inhibition was measured as 9 mm for the dose of 200 mg formulation for gram-positive bacteria,...
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Posted by admin on Jan 11, 2018 in |
Identification of natural marine bioactive molecules and their therapeutic potential is valuable considering that there are reduced chances of undesirable side effects. This study was designed to isolate and purify the polysaccharides from E. intestinalis and evaluate its anti-cancer effect on human hepatocarcinoma cell lines (Hep3B). Polysaccharides from Enteromorpha intestinalis were isolated and purified. Preliminary characterization was done using UV-Vis spectrum and FTIR analysis. Total sulfate content of the polysaccharides was determined. MTT assay, AO / EB and DAPI staining were done to determine the cell growth pattern and nuclear changes effected by polysaccharides on Hep3B cells. Effect of polysaccharides on mitochondrial membrane potential and ROS production on Hep3B cells were evaluated using Rhodamine 123 and DCHF-DA staining. Results showed that polysaccharides from E. intestinalis were rich in sulfate groups (sPS). Cell viability, assessed in-vitro by MTT assay showed that the number of viable cells was less than 50% when exposed to concentrations exceeding 1 mg/ml of sPS. Cell viability and nuclear integrity assays depicted cells with compromised membrane...
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