ANTIBACTERIAL ACTIVITY AND HPLC ANALYSIS OF THEVETIA PERUVIANA LEAF EXTRACTSAbstract
Thevetia Peruviana (or Cascabela thevetia) belongs to the family Apocynaceae and commonly called as Yellow Oleander. The leaves of this significant medicinal plant were subjected to antibacterial investigation and HPLC analysis. Hexane, acetone, and methanol were used as organic solvents. Four different concentrations (200 mg/ml, 100 mg/ml, 50 mg/ml and 25 mg/ml) of each extract i.e. hexane, acetone and methanol were prepared for antibacterial activity by dissolving them in 100% DMSO. The leaf extracts were subjected to test the antibacterial activity by agar well diffusion method. The methanol extract of leaves showed significant antibacterial activity against Pseudomonas aeruginosa and poor activity against Micrococcus luteus and Shigella flexneri. Acetone extract showed moderate activity against the tested micro-organisms viz. Pseudomonas aeruginosa, Micrococcus luteus and Shigella flexneri. Hexane extract showed moderate activity against Micrococcus luteus and poor activity against Pseudomonas aeruginosa and Shigella flexneri at different concentrations. HPLC analysis of methanol leaves extract was performed which showed that plant leaf contains different types of phytosterols. Thevetia peruviana leaves a promising possibility of developing a new drug of pharmaceutical interest.