FORMULATION AND EVALUATION OF GEL CONTAINING MANGIFERA INDICA LEAVES EXTRACT FOR ANTI-BACTERIAL ACTIVITYAbstract
In the present study, an attempt was made to formulate ethosomal gel containing leaf extract of Mangifera indica, and was evaluated for antibacterial activity against gram-positive and gram-negative organisms. Different formulations of ethosomes using lecithin, cholesterol, and ethanol were prepared using Mangifera indica herbal leaf extracts. Carbopol 940 was used to prepare ethosomal gel. The entrapment efficiency of ethosomes was 65.1% – 96.54% and the average vesicle size was 920 nm. Three formulations of different doses (100, 200 and 300 mg) were selected based on entrapment efficiency and drug release. Prepared gels were then evaluated for physicochemical characteristics and drug content. The antibacterial activity was done using agar well diffusion method for the zone of inhibition. The pH of the gel formulations was found to be in the range of 5.4 – 6.2 and viscosities between 2250 – 2399 centipoises. The drug content of gels ranged between 74.67 – 82.31%. The zone of inhibition was measured as 9 mm for the dose of 200 mg formulation for gram-positive bacteria, Bacillus subtilis and 13 mm for gram-negative bacteria Pseudomonas aeruginosa. Norfloxacin and Ciprofloxacin were used as standard. It was found that the ethosomal gel of Mangifera indica herbal extract FN2 (Formulation 2, 200 mg) was found to possess greater zone of inhibition when compared to the other two formulations. From the study, it can be concluded that ethosomal gel served as an efficient drug delivery system for herbal extract with potential antibacterial activity.
P. S. Rao *, B. T. Sundari and S. Kalva
Sri Venkateshwara College of Pharmacy, Madhapur, Hyderabad, Telangana, India.
21 July 2017
23 August 2017
17 September 2017
01 January 2018