CHARACTERIZATION OF PHYTOCHEMICAL AND ANTIOXIDANT PROPERTIES OF CITRUS AURANTIFOLIA (KAGOJI LEBU) AVAILABLE IN BANGLADESH AND ITS PROTECTIVE EFFECTS AGAINST EXPERIMENTALLY INDUCED MYOCARDIAL INFARCTION IN RATSAbstract
Plants and fruits have medicinal purposes, and they are being used long before the prehistoric period. Among the different varieties of citrus fruits, Citrus aurantifolia (Kagoji labu) is widespread in tropical and subtropical regions around the World. It is one of the major citrus fruits and widely consumed, but there is limited evidence about its health-promoting properties. In the present study, we evaluated the phytochemical and antioxidant properties of this citrus fruit since phytochemicals through anti-oxidant properties have played a significant role in human health protection and treatment of many diseases. Polyphenol, flavonoid, tannin and reducing sugar contents were found 4.68 ± 0.36 g GAE / 100 g extract, 615.38 ± 17.75 mg CE / 100 g extract, 12.5 ± 6.25 g TAE / 100 g extract and 51.69 ± 7.50 g GE / 100 g extract, respectively. Significant antioxidant activities of C. aurantifolia via 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and ferric reducing antioxidant power (FRAP) were detected. Earlier studies have reported that almost all species of citrus fruits have significant antioxidant properties and are effective against stress-induced ulcers, cancer, and other chronic diseases. Therefore, next, we asked about the protective effects of C. aurantifolia extract on isoproterenol (ISO) induced myocardial infarction (MI). Subcutaneously injected ISO (a well-known chemical to produce MI in experimental animals) causes a significantly (p<0.01) increase of serum concentrations of lactate dehydrogenase (LDH) and creatine kinase-MB (CK-MB), two myocardial cell-specific enzymes which are increased in blood after MI, indicating ISO injection produced MI. Hematoxyline and eosin (H and E) staining of the heart showed relatively thin and abnormal myocardial cell fibers in ISO injected rats when compared with normal control. Treatment of rats with C. aurantifolia (1000 mg/kg body weight) followed by ISO injection significantly (p<0.01) decrease the serum concentrations of LDH and CK-MB, indicating the protective effects of this fruit against ISO induced MI. H and E staining also showed treatment with C. aurantifolia followed by ISO injection causes the myocardial cell fibers to remain as thick as normal control rats. Together all data suggests that C. aurantifolia are rich in phytochemical properties, and it has protective effects against ISO induced MI.
A. Kabir, R. Karim, M. Y. Ali, M. I. Khalil and M. I. Hossain *
Laboratory of Preventive and Integrative Biomedicine, Department of Biochemistry and Molecular Biology, Jahangir Nagar University, Savar, Dhaka, Bangladesh.
28 November 2019
23 December 2019
26 December 2019
31 December 2019