Posted by admin on May 31, 2021 in |
Objective: Stem Bark, Root, and fruit of Ziziphus oenoplia (Rhamnaceae) has been used in traditional health systems to treat diabetes and many more disorders in southern Asia as India, Sri Lanka, and Burma. However, the anti-oxidant and hypoglycemic potential of the fruit of this valuable plant is not scientifically validated till date. The aim of the present study is to evaluate in vitro free radical scavenging and hypoglycemic effect of ethanol extracts and solvent fractions of Ziziphus oenoplia fruit. Methods: The ethanol extracts and three solvent fractions of Ziziphus oenoplia fruit were evaluated at different concentration (20- 640 µg/ml) for free radical scavenging activity by using in-vitro DPPH method and different concentration (25-800 µg/ml) for hypo-glycemic potentials by using α – glucosidase and α-amylase enzymes (intestinal enzymes which slow the digestion of carbohydrates and delay glucose absorption) inhibitory activity. Results: The outcome of present study indicates that maximum anti-oxidant activity was produced by ethanol crude extract (87.66±1.54% Inhibition at 640 μg/mL) with IC50 value 2.72±0.62 followed by chloroform fraction...
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Posted by admin on May 31, 2021 in |
The methanolic rhizome extract of Pseudodrynaria coronans (MEPC) was assessed for its preliminary phytochemical constituents, anti-nociceptive and anti-inflammatory activity. Acute toxicity of the extracts was tested following the OECD – 425 guidelines and after giving MEPC at the dose of 2000 mg/kgb.w indicating that the LD50 is higher than the given dose. Two doses 200 and 400 mg/kg bw. were selected for all the experiments. Hot plate and Tail immersion methods showed that the extract was statistically significant (p<0.05 and p<0.01). Different concentrations of the MEPC showed significant inhibition of protein denaturation and membrane stabilization. Also, the MEPC exhibited significant inhibition of carrageenan-induced paw oedema (p<0.01) and cotton pellet-induced granuloma (p<0.05 & p<0.01) inflammatory responses in the...
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Posted by admin on May 31, 2021 in |
Objective: To investigate the Pharmacognosy as well as the Antioxidant, Anti-inflammatory potential of the Katha powder. Methods: The Coarsely dried chips of Acacia catechu heartwood were treated with 10% hydro-alcoholic solution to obtain Katha as the final product. The powdered Katha was standardized via pharmacognostic parameters. This Kathapower is showing good solubility in hot water, having astringent in the taste. The powder microscopy of the Katha powder is to be demonstrated fragments of acicular crystals, fibers and bordered pitted vessels. Katha powder antioxidant potential is to be accessed by using the DPPH assay and NO Scavenging assay by using ascorbic acid as a standard drug. Further, the Katha powder is to be subjected for the assessment of its anti-inflammatory potential by use of Heat-induced hemolysis as well as the Hypotonicity-induced hemolysis approach by the use of aspirin or diclofenac sodium as a standard drug. Results: Microscopical investigations were showed that Katha showing the presence of fragments of acicular crystals, fibers, and bordered pitted vessels. In-vitro study shows that the...
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Posted by admin on May 31, 2021 in |
The spread of infectious diseases in the world is a major challenge for health institutions and pharmaceutical industries. The current trends of multi-drug resistance to the available synthetic drugs or antibiotics among emerging and re-emerging bacterial pathogens lead to serious risks. It is therefore necessary to search for new antimicrobial agents that are better, cheaper and without side effects for treating these infectious diseases, especially in developing countries. In this study, phytochemical composition and antimicrobial activities of aqueous and methanol extracts of stem-barks of Mangifera indica were investigated. Standard methods were employed to screen for the presence of phytochemicals. Agar well diffusion method was used to determine the antimicrobial effects of aqueous and methanolic extracts of M. indica stem-bark against selected bacterial (Shigella sp, Staphylococcus sp, Escherichia coli, Vibrio sp) and fungi (Penicillium sp, Yeast, Mould) isolates. Phytochemical results showed the presence of active pharmacological components such as tannins, saponins, glycoside, flavonoid, terpenoid, alkaloids, and steroid. Methanol extract demonstrated the highest activity of bacterial (Staphylococcus sp with 15.4 ±...
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Posted by admin on Apr 30, 2021 in |
Objective: Plants live on a planet with days and seasons, affecting their phytoconstituents. The challenge is that availability of active principles in medicinal plants changes by seasonal fluctuations, so their dose pattern for therapeutic efficacy also get influenced. The best duration for the harvesting of specific secondary metabolites for better yield is not fixed. Seasonal impact show changes in important constituents like polyphenol, flavonoids, glycosides, alkaloids, essential oil, etc. Late summer is the best collection time for essential oil components. Winter and rainy are the best season for other secondary metabolites. Methods: The selected plant Azadirachta indica A. belongs to the alkaloidal category with having anti-diabetic activity. Plants were evaluated for pharmacognostic study, which includes macroscopic and microscopic evaluation, determining physicochemical parameters systematically. HPTLC fingerprinting for nimbin was done. The study was performed for plant material with three different seasons, and the best results were analyzed. Results and Conclusion: All the plants showed correct taxonomy with specific morphological, microscopical, and physicochemical parameters, which is helpful for the standardization of...
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