IMPACT OF SEASONAL VARIATIONS ON NIMBIN ALKALOID FROM AZADIRACHTA INDICA A.Abstract
Objective: Plants live on a planet with days and seasons, affecting their phytoconstituents. The challenge is that availability of active principles in medicinal plants changes by seasonal fluctuations, so their dose pattern for therapeutic efficacy also get influenced. The best duration for the harvesting of specific secondary metabolites for better yield is not fixed. Seasonal impact show changes in important constituents like polyphenol, flavonoids, glycosides, alkaloids, essential oil, etc. Late summer is the best collection time for essential oil components. Winter and rainy are the best season for other secondary metabolites. Methods: The selected plant Azadirachta indica A. belongs to the alkaloidal category with having anti-diabetic activity. Plants were evaluated for pharmacognostic study, which includes macroscopic and microscopic evaluation, determining physicochemical parameters systematically. HPTLC fingerprinting for nimbin was done. The study was performed for plant material with three different seasons, and the best results were analyzed. Results and Conclusion: All the plants showed correct taxonomy with specific morphological, microscopical, and physicochemical parameters, which is helpful for the standardization of drugs. Extracts showed the presence of alkaloids, terpenes, flavonoids, steroids, phenolics, saponin, and carbohydrates. HPTLC fingerprinting confirmed the presence of nimbin in the plant extracts. Seasonal variations occur in plant constituent shows best collection period. Current research aims to focus on the best possible season for the harvesting of some pharmaceutically important plant materials.
G. Palshikar and P. S. Pandiyan *
School of Pharmacy, Sathyabama Institute of Science & Technology, Deemed to be University, Chennai, Tamil Nadu, India.
26 March 2021
25 April 2021
29 April 2021
30 April 2021