Posted by admin on Apr 30, 2025 in |
The complication most feared by diabetics remains the diabetic foot, due to the risk of amputation. Terminalia ivorensis belongs to the Combrétacée family and is rich in secondary metabolite products. Studies have shown that Terminalia ivorensis has antifungal potentiaĺ and is traditionally used to treat wounds. Topical application based on Terminalia ivorensis on wounds of rats rendered diabetic showed a rapprochement between the results given by this application and L-mesitran® used as a reference molecule. The aqueous extract at different concentrations (500, 1000 mg/ml) showed significant healing activitý after topical administration in...
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Posted by admin on Apr 30, 2025 in |
Ficus mucuso Welw. ex Ficalho (Moraceae) is a plant used to treat the after-effects of strokes in Côte d’Ivoire. The aim of the present work is to characterize the physicochemical properties of the stem bark of this plant drug, evaluate its anti-inflammatory properties and determine the presence of minerals of interest in post-stroke treatment. Minerals play a crucial role in stroke prevention and management. Physicochemical tests revealed that Ficus mucuso stem bark has a pH of 5.81, a fairly low average water content (7.98%), a relatively high average dry matter content (92.02%), an ash content of 9.21%, and is rich in iron, potassium, magnesium and calcium, important minerals in stroke prevention and management. EAq has the highest concentrations of Potassium (160±11.3 mg/L) and Calcium (11±1.05 mg/L), while EEt has the highest concentrations of Magnesium (2.3 ± 0.12 mg/L) and Iron (1.38 ± 0.04 mg/L). Ficus mucuso stem ecore extract also has anti-inflammatory activity. Aqueous and ethanolic extracts of Ficus mucuso stem bark induce inhibition of protein denaturation at concentrations...
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Posted by admin on Apr 30, 2025 in |
This study is aimed at investigating/elucidating the molecular structure and the biochemical mechanism of anti-inflammatory action of an isolated but yet unknown bioactive compound CA1 from Crateva adansonii stem bark extract. Qualitative phytochemical tests on the isolated bioactive anti-inflammatory compound gave positive reaction to terpenoid only, while the structural elucidation indicates that CA1 is (3β)-lup-20(29)-en-3-ol, a pentacyclic triterpene with molecular weight of 426.70 g, and molecular formular of C30H50O. The molecular structure of this pentacyclic triterpene was established using nuclear magnetic resonance (NMR), ultra-violet/visible spectrophotometer, infra-red, gas and mass spectrophotometers. The (3β)-lup-20(29)-en-3-ol was subjected to in-vivo anti-inflammatory activity study using egg-albumin induced rat hind paw edema method. However, (3β)-lup-20(29)-en-3-olwas investigated for its selective COX-1 and COX-2 inhibitory activities. An in-vitro model of inflammation using a colourimetric COX (ovine) inhibitor screening assay method was used as a model to investigate the effect of CA1 on PGE2 production. (3β)-lup-20(29)-en-3-ol at the tested concentrations significantly (P < 0.05) and selectively inhibited the inducible form of cyclooxygenase, cyclooxygenase-2 enzyme, but exhibited no inhibitory...
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Posted by admin on Apr 30, 2025 in |
Ginger (Zingiber officinale) is one of the most studied medicinal plants, and its bioactive compounds, especially alkaloids, have been demonstrated to possess intense pharmacological activities. We aimed to identify and quantify the major alkaloids present in ginger rhizomes, using phytochemical screening, spectrophotometric analysis and high-performance liquid chromatography (HPLC). The authenticated fresh ginger rhizomes were processed by methanol extraction, which produced 0.704% (w/w) crude extract. In qualitative tests (Mayer’s, Wagner’s, and Dragendorff’s reagents) alkaloids were confirmed; and in spectrophotometric tests, total alkaloid content was determined (18.45 ± 1.27 mg of atropine equivalents/g of extract). In addition, we also analyzed constituents of ginger and found four principal alkaloids of HPLC as follows: 6-gingerol (10.73 ± 0.85 mg/g), 6-shogaol (10.42 ± 0.47 mg/g), 8-gingerol (3.81 ± 0.32 mg/g) and 10-gingerol (3.45 ± 0.43 mg/g). The calibration curves (r²>0.95) for these compounds were excellent, indicating reliability of the method. The high contents of 6-gingerol and 6-shogaol are associated with their well-known anti-inflammatory, antioxidant and anticancer effects, whereas those of 8- and 10-gingerol add to those are presented...
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Posted by admin on Apr 30, 2025 in |
Keratinases of microbial origin are a group of protease enzymes with specific characteristic feature of proteolytic cleavage of keratin resulting in the generation of soluble peptides and free amino acids. They play important roles in conversion of world richest proteinous solid-waste‘’ keratinaceous waste’’ into value-added products. This current research work aimed at isolation, purification, characterization and optimization of keratinase isolate from Aspergillus niger Strain (ND44) obtained from human hair macerated in salt medium, for its enzymatic properties in the degradation of trichocytic α-keratin(a fibrous structural protein that resist most proteases degradation based on its stabilized intra-molecular disulphide and hydrogen bonding), while gene sequencing and phylogenetic tree gave expanded details on the structural identity of the isolated Aspergillus niger Strain (ND44). High keratinolytic activity exhibited by this microbial keratinase at high temperature up to 90⁰C confirms it as a hyperthermophilic enzyme, with non-optimums pH peaks at 5.5 and 9.00that spread across both acidic and alkaline regions. The optimum pH, temperature and best co-factors for this human hair keratinase activities obtained...
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