Posted by admin on Dec 31, 2020 in |
The aim of this study was to evaluate antisecretory and antioxidant activities of the leaves aqueous extract of Boscia angustifolia (LAEBA) on gastric ulcers induced in male rats. LAEBA at the doses of 125, 250, and 500 mg/kg were tested on three models of gastric ulcers, namely: pylorus ligation, pylorus ligation + histamine, and pylorus ligation + carbamylcholine. Some parameters of oxidative stress (malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (GSH)) were evaluated in stomach’s homogenates. The qualitative phytochemical screening of LAEBA was carried out. Antisecretory screening showed that LAEBA at 500 mg/kg significantly decreased (p<0.01) gastric acidity (8.06 mEq/l) compared to the negative control (14.07 mEq/l), with a significant reduction (p<0.001) of gastric volume (1.30 ml) compared to the negative control (5.12 ml), as well as a reduction of pepsin activity (64.64%). These variations were similar to pylorus ligation + histamine and pylorus ligation + carbamylcholine models. LAEBA at all the doses induced a significant reduction (p< 0.05) of ulcerated surface correlated with an increase...
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Posted by admin on Dec 31, 2020 in |
Genus Memecylon belonging to the family Melastomataceae is a large shrub or a small tree native to Western Ghats of India. Memecylon species are reported to show various biological activities and are also used as medication in herpes virus infection. RNases and proteases are involved in the replication of herpes simplex virus (HSV). The present study was taken up to screen RNase and protease (trypsin and thrombin) inhibitors in different solvent extracts of Memecylon species viz., M. umbellatum, M. malabaricum, M. wightii, M. edule and M. talbotianium. The methanol extract of the leaves of Memecylon species was observed to strongly inhibit RNase, trypsin, and thrombin functions in a dose-dependent manner. The percent inhibition of RNase activity ranged from 53-84% in 50 ug/mL of plant extracts. The highest inhibition was observed in M. malabaricum and M. wightii for RNase. Similarly, for trypsin the percent inhibition ranged from 55-68% in 1 mg/mL of plant extracts. Highest inhibition was observed in M. malabaricum, M. wightii, and M. talbotianium. For thrombin the percent...
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Posted by admin on Dec 31, 2020 in |
Cervical cancer is a highly prevalent global disease among females and currently available treatments are nevertheless restricted and have substantial adverse effects. Plants may be a rich source of molecules with therapeutic potentials, such as Campomanesia aureas O. Berg, Myrtaceae, a native species from the South of Brazil with few data regarding its biological effects. In this study, we investigated the chemical composition and antineoplastic potential in-vitro of the essential oil (EO) of C. aureas leaves in human cervical cancer cells (SiHa), as well as its effect in the viability of non-tumoral cells (HaCat). CG-MS analysis reveals that the EO is rich in oxygenated sesquiterpenes and monoterpenes and MTT colorimetric assay shows that it significantly inhibits the viability of the SiHa cell line at different concentrations. Besides, the EO reduced the colony-forming capacity of the tumor cell line, and the ratio between the IC50 values of cell lines demonstrated a promising selectivity index for C. aurea EO. Therefore, the EO could be a potential source of new anticancer...
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Posted by admin on Dec 31, 2020 in |
Objective: The aim of this study was to evaluate the anticancer activity of a series of six structurally related [1, 4]-Benzoquinone monooximes and to investigate structure-activity relationships (SAR) of these compounds. Materials and Method: The mono-oximes obtained by oximination of para-benzoquinones 1-4 had their structures confirmed by ¹H NMR, 13C NMR. The cytotoxic study was assessed by the MTT assay on three tumor cell lines: HL60 (promyelocytic leukemia), NCI-H292 (lung cancer), and MCF-7 (breast adenocarcinoma). Results: Six oximes were synthetized 2 – methyl-[1,4]-benzoquinone oxime tosylate (I), 5-isopropyl-2-mehyl-[1,4]-benzoquinone oxime tosylate (II), 5-isopropyl-5-mehyl-[1,4]-benzoquinone oxime tosylate (III), 5-isopropyl-2-mehyl-[1,4]-benzoquinone oxime (IV), 2,5-dimethyl-[1,4]-benzoquinone oxime tosylate (V), 2-methyl-[1,4]-benzoquinone oxime tosylate (VI). Compounds II to V showed significant activity against all cell lines; while compound I and VI presented low or no activity. Conclusion: These experimental data lead to the conclusion that oximes (II –V) may be used as potential starting structures for the design of novel anticancer...
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Posted by admin on Oct 31, 2020 in |
The main objective of this present work is to evaluate the anthelmintic activity of crude alcohol extracts of the flowers of kachnar (Bauhinia variegata) against Pheretima posthuma as a test worm. The activity was assessed by worm motility assay, which involved the determination of time of paralysis and death of worms. The preliminary phytochemical study of the alcoholic extract was also carried out. The alcoholic extracts exhibited a dose-dependent (25, 50, and 100 mg/ml) inhibition of spontaneous motility and time of death of the worms. The alcoholic extract was found to be effective; the activity was comparable with the standard drug Piperazine citrate and water as a control....
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