ISOLATION AND PURIFICATION OF ANTI-TUMOR ENZYME L-ASPARAGINASE FROM SOIL ISOLATESAbstract
16 different bacteria were isolated from soil collected from and near Aurangabad city. All of the isolates were screened for L-asparaginase activity using modified M-9 medium. Spectro-photometric determination of L-asparaginase was done, and it was partially purified using ammonium sulphate precipitation. Out of the bacterial species screened only two were found to be producers of L-asparaginase. The Biochemical identification revealed both the species to be of the genus Bacillus. The crude extract showed a low enzyme activity as compared to partially purified extract. Both the isolates gave a maximum enzyme activity at pH 8 and a temperature of 40 °C. The genus Bacillus has become a promising candidate for the production of L-asparaginase from the other reported bacteria.