Posted by admin on Jan 11, 2018 in |
The study aims to standardize a vaginal herbal formulation consisting of stem barks of Ficus glomerata Roxb. and Symplocos racemosa Roxb. Since they are used as Ayurvedic medicine and described in ancient text Charak Samhita as astringent, anti-inflammatory and treatment of vaginitis/leucorrhoea. This new herbal formulation is likely to exhibit significant antimicrobial action due to the presence of phenolic and flavonoid compounds. The extractive value was 1.64 and 1.70% w/w for aqueous and alcoholic extract. Total Phenol Content was 225.67 and 220.00 µg Gallic acid equivalent/mg for aqueous and alcoholic extract. Fifteen types of mucoadhesive tablets weighing 550, 700 or 900 mg each were prepared using combinations of research drug, Carbopol and Hydroxy-Propyl-Methyl Cellulose as polymers, talc as a glidant, magnesium stearate as a lubricant and Micro-Crystalline Cellulose and Lactose monohydrate as diluents. Similarly, five types of effervescent mucoadhesive tablets using sodium bicarbonate and citric acid in 3:1 ratio were prepared. Apart from weight, thickness, hardness, and friability of each group, the surface pH, swelling index and muco-adhesive force...
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Posted by admin on Jan 11, 2018 in |
Objective: To evaluate the chemical composition, antioxidant and antiproliferative activities of Hyptis pectinata aerial part aqueous extract (HYP). Method: Chemical profiling using high-performance liquid chromatography with UV diode array detector (HPLC-UV-DAD), in-vitro antioxidant and anti-proliferative studies were done. Antioxidant and antiradical activities of HYP was investigated using 3-(4,5-dimethylimidazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and DPPH radical scavenging assay. Result: The present study revealed several metabolites. HPLC analysis gave caffeic acid (13.92%), rutin (7.89%) and ferulic acid (5.44%) as some of the bioactive constituents. HYP showed antioxidant activity in both the DPPH and MTT assay. HYP exhibited antioxidant activity against DPPH and MTT from the first dilution at 10 mg/ml up to the ninth dilution at 0.08 mg/ml in a 2-fold dilution signifying the presence of antioxidant compounds at those concentrations. At 1 mg/ml to 32 mg/ml, HYP dose-dependently and significantly (P < 0.001) inhibited Sorghum bicolor seed radicle growth throughout 24-72 h compared to the negative control. Conclusion: Hyptis pectinata aerial part aqueous extract (HYP) possessed antioxidant and antiproliferative activities, hence providing...
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Posted by admin on Nov 30, 2017 in |
Aqueous and ethanol ethyl acetate chloroform-methanol extracts from the leaves of Ricinus communis were investigated for their anthelmintic activity against Pheretima posthuma and three concentrations (50, 75 and 100 mg/ml) of each extract were studied for activity, in which paralysis and death time of the worm. Both the extract exhibited remarkable anthelminthic activity at all three concentrations. Albendazole suspension is used as standard reference (20 mg/ml) and distilled water as control. Therefore the aqueous and ethanol methanol extract of leaves of Ricinus communis has been demonstrated for the first...
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Posted by admin on Nov 30, 2017 in |
Background: There are very less therapeutic and reliable liver protective drugs in modern medicine to prevent and treat the liver injury caused due to drugs. Traditionally Solanum xanthocarpum Schradt. (SX) and Wendl. and Juniperus communis Linn. (JC) have been used since long for their hepatoprotective effect. The current study was carried out to explore the hepatoprotective role of SX and JC against liver toxicity in rodents induced by carbon tetrachloride (CCl4). Materials and Methods: The antioxidant potential of hydroalcoholic extract of SX and JC were investigated by using established DPPH in-vitro assay method. The hydroalcoholic extract of SX and JC at doses (200 and 400 mg/kg b.w.) were studied for their liver protective activity against hepatic damage induced by CCl4 in Wistar rats. An assay of the oxidative stress parameters, alkaline phosphatase, transaminase, total bilirubin, albumin and liver histopathology were performed to evaluate the hepatoprotective activity of SX and JC. The assay results were introduced as the standard error ofthe mean (SEM) for each group. Results: The ethanolic extract...
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Posted by admin on Nov 30, 2017 in |
The anti-inflammatory and antioxidant activities of the methanol extract of Amomum masticatorium rhizome were observed in various experimental models. Phytochemical constituents like phenols and flavonoids were also estimated. The antioxidant activity was evaluated by DPPH and superoxide radical scavenging assays. The extract showed significant antioxidant activity. The in-vitro anti-inflammatory activity of the extract was investigated by various methods viz. albumin denaturation, membrane stabilization, and proteinase inhibitory assays. Results showed that the extract exhibited significant anti-inflammatory activity by inhibiting the heat-induced albumin denaturation with IC50 value 34.15 ± 0.404µg/ml, effectively stabilized RBC membrane with an IC50 value of 51.47 ± 1.56 µg/ml and significantly inhibited proteinase activity with an IC50 value of 52.8 ± 0.61µg/ml. In-vivo activity was evaluated in carrageenan and formalin-induced paw edema test in mice and compared with that of standard drug diclofenac. In both models, the extract showed a significant reduction in edema. The extract at 200 mg/kg b. wt showed a significant reduction in carrageenan-induced paw edema at a 3rd h and chronic edema on...
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