Posted by admin on Mar 15, 2018 in |
This study was aimed to maximize the benefits of the use of by-products (oil cake) of olive fruits and black seeds after extraction of their fixed oils, rather than their use as animals feed or landfilling or composting. The cake of both seeds was assessed by HPLC for their main bioactive marker compounds (thymoquinone for black seed and oleuropein for olive fruit), to choose the best method for extraction, determination of phenolic contents and in vitro antioxidant activity (DPPH and reducing powers, FRAP assays). Both by-products were also assessed for their cytotoxicity against four human breast cell lines, three of them are cancerous (MCF7, MDA-MB-213, Vcr-R), and a non-cancerous one (epithelial type) but immortalized by telomerase (hTERT-HME), in addition to a human hepatoma cell line (HepG2). Also, both wastes were subjected for in-vitro CDC25s phosphatase inhibition assay on three isoforms (A-C) and for in-vivo immunomodulatory effects. In conclusion, the results of this study showed the interest of cumulating different biological approaches exploring various physiological mechanisms and showed the utility...
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Posted by admin on Feb 5, 2018 in |
The objective of this research is to evaluate the preliminary phytochemical analysis and antibacterial activity from the medicinal plant of Hugonia mystax of Dindigul district, Tamil Nadu. The leaves of plant Hugonia mystax was subjected to Soxhlet extraction process by using solvent ethanol. Preliminary phytochemical analysis of ethanol extract of Hugonia mystax leaves showed the presence of carbohydrate, flavonoids, phenolic groups, saponins, steroids, tannins, and terpenoids. In-vitro antibacterial activity was performed by the plate hole diffusion method in MH agar medium. The bacteria used for the antibacterial study were Bacillus lichenformis (NCIM 2468), Brevibacterium luteum (ATCC 15830), Escherichia coli (ATCC 11775), Flavobacterium devorans (NCIM 2581), Klebsiella pneumonia (ATCC 11229), Micrococcus flavus (NCIM 2984), Micrococcus luteus (NCIM 1207). Ethanol extract of Hugonia mystax exhibited the best result of the maximum zone of inhibition observed against gram-positive as well as gram-negative bacteria. The anti-bacterial activity revealed the medicinal potential of Hugonia mystax to develop a drug against human...
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Posted by admin on Feb 5, 2018 in |
Objective: The study was designed to explore the anti-oxidant, anti-microbial and anti-inflammatory activity of Tecoma capensis Thumb. (T. capensis) whole plant extract. Material and Method: Antioxidant and free radical scavenging activity of extract of ethanolic extract of Tecoma capensis was investigated against 2, 2 Diphenyl-1-picrylhydrazyl (DPPH) assay, superoxide radical and hydroxyl radical scavenging activity compared with that of standard (ascorbic acid) and antimicrobial activity was evaluated against clinically important bacterial strain those were Bacillus subtilis, Staphylococcus aureus (gram +ve), Escherichia coli, Proteus vulgaris (gram -ve) and two fungal strain Aspergillus niger, Candida albicans using cup plate method. Anti-inflammatory activity was evaluated by carrageenan-induced rat paw edema method by the oral administration of EETC (100, 250 mg/kg). The data were statistically analyzed by ANOVA. Results: The IC50 values were determined by radical scavenging activity of EETC by DPPH (21 ± 0.06), superoxide radical (37.2 ± 0.04) and hydroxyl radical scavenging activity (12 ± 1.64). The average zone of inhibition for EETC against S. aureus (15 ± 0.816) and E. coli...
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Posted by admin on Feb 5, 2018 in |
Dregea volubilis (L.f.) Benth. Ex Hook. f. is a large twinning shrub belonging to the family Asclepiadaceae. The leaf is amphistomatic with tetracytic, anomocytic, paracytic stomata. The sides of the leaf in the surface are straight to curved, surface striated on either side. The mesophyll is interspersed with sphaerocrystalliferous idioblasts. Midvein consists of a single arcuate vascular bundle, bicollateral, conjoint, endarch. In T. S. petiole is oval, adaxially grooved at center and with two lateral adaxial ridges, consisting of a large arc shaped vascular bundle at the centre with a 2 small spherical vascular bundles in adaxial...
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Posted by admin on Feb 5, 2018 in |
Antigonon leptopus Hook & Arn (family Polygonaceae), also called: Chain of love, Queen’sWreath, and Mexican creeper is a fast-growing vine with stems reaching up to 20 feet long. It has heart-shaped, green leaves climbing by tendrils which wrap around many types of supports. It is native to Mexico and commonly found in tropical Asia, Africa, the Caribbean, and the Americas. It possesses anticoagulant activity, analgesic, anti-thrombin, anti-inflammatory, anti-diabetic and antidepressant activities. Aerial parts of the vine have been used as hepatoprotective and for spleen disorders. During the present study, LC/MS experiment was conducted on the methanolic extract of the aerial parts of the vine. Results revealed the identification of total fifty compounds; flavonoids, phenolics, anthocyanins, and glycosides. Compounds were characterized by their retention times, mass spectra with comparison to online mass bank database. From the results, Antigonon leptopus can be incorporated in pharmaceutical supplements used to aid free radical-mediated disease; cancer, diabetes, inflammation and liver...
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