Posted by admin on Feb 1, 2014 in |
Antioxidant effectiveness of indigenous medicinal plant C. roseus leaves extracts and fractions with solvents of different polarity (ethanol, methanol, acetone, hexane, butanol, and water) were assessed for DPPH radical scavenging activity. The C. roseus extracts and fractions contained appreciable levels of the antioxidant activities of leaves of C. roseus at various concentrations. The leaves of C. roseus showed good antioxidant activity (81.70%). The phytochemical analysis of the C. roseus shows the presence of alkaloids, terpenoids, steroids, flavonoids, and other plant secondary metabolites. GC-MS result shows that the important secondary metabolites and their derivatives present in C. roseus leaves extract. The results of the present comprehensive analysis demonstrated that C. roseus extracts and fractions are a viable source of natural antioxidants and might be exploited for functional foods and nutraceutical...
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Posted by admin on Feb 1, 2014 in |
Flurbiprofen is a new non-steroidal anti-inflammatory agent, one of the propionic acid groups, which has significant anti-inflammatory, analgesic, and antipyretic properties. Clinically, it is used for the treatment of rheumatoid arthritis, degenerative joint disease, osteoarthritis, ankylosing spondylitis, acute musculoskeletal disorders, low back pain, and allied conditions. In this study, the first-order derivative spectrophotometry method was developed for the determination of flurbiprofen in pharmaceutical preparations. In the first derivative spectrophotometry, absorbance values were measured at 213, 233 and 260 nm. Parameters such as linearity, precision, accuracy, specificity, stability, limit of detection and limit of quantitation were studied according to the International Conference on Harmonization Guidelines. A calibration curve was linear between the concentration ranges of 1-14 μg ml-1. Within- and between-day precision values for flurbiprofen were less than 4.95%, and accuracy (relative error) was better than 3.67%. The mean recovery value of flurbiprofen was 100.9% for pharmaceutical preparations. The developed method was successfully applied to tablet formulations and the results were compared statistically with each...
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Posted by admin on Feb 1, 2014 in |
Uvaria narum Wall (Annonaceae) is described as a hepatoprotective in Ayurveda, the Indian system of medicine. However, there are no scientific reports in the modern literature regarding its usefulness as a hepatoprotective agent. The present study was carried out to evaluate the hepatoprotective activity of alcoholic (UNAL) and aqueous (UNAQ) extracts of leaves of Uvaria narum Wall in paracetamol-induced hepatotoxicity in rats. Alcoholic and aqueous extracts at doses of 200 and 400 mg/kg were administered orally once daily for 7 days. The hepatoprotective activity was assessed using various biochemical paradigms related to oxidative stress like increased liver lipid peroxidation, elevation of serum marker enzymes (AST, ALT, ALP, and LDH), decreased non-enzymatic (glutathione) and enzymatic antioxidants (CAT and SOD) activity and reduction of total proteins in liver homogenate along with histopathological studies of liver tissue. Treatment with UNAL and UNAQ extracts prevented the elevation of serum biomarkers AST, ALT, ALP, LDH, TB, and depletion of TP dose-dependently. The depletion of antioxidants GSH, SOD, and CAT and increased MDA formation in...
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Posted by admin on Feb 1, 2014 in |
Seven different Cyanobacterial species belonging to 4 genera were isolated from the alkaline Lake Lonar, situated in the Buldhana district of Maharashtra. The phycocyanin producing ability of all the isolates was screened. All the isolated alkaliphilic cyanobacteria showed more than 10 mgL-1 of phycocyanin. From the seven cultures tested, Synechocystis aquatilis showed the highest production of phycocyanin followed by Phormidium laminosum and Oscillatoria amphibian, respectively. 2, 2 diphenyl-1-picrylhydrazyl (DPPH) assay was performed for screening the highest percentage of radical scavenged was shown by Spirulina platensis (40.28), followed by Synechocystis aquatilis (36.32), Phormidium laminosum (34.80) respectively. All the three Cyanobacterial cultures were put under salt stress of 100 to 1000mM. An increase in the % radical scavenging activity was seen when all the three alkaliphilic cyanobacteria were under salt stress. 22% increase in the radical scavenging activity was seen in Spirulina platensis, while 16% increase was observed in the other two cultures. Thus, the antioxidant potential of the alkaliphilic Cyanobacteria isolated from Lonar Lake was...
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Posted by admin on Feb 1, 2014 in |
Lagenaria siceraria was commonly known as Bottle gourd Syn. Doodhi, Syn. Lauki (Hindi) Kadoo (Marathi) which is official in Ayurvedic Pharmacopoeia. Preliminary phytochemical screening of the crude extracts revealed the presence of carbohydrates, proteins, etc. and secondary metabolites like saponins, glycosides, alkaloids, etc. in all the extracts but tannins were found absent in ethanolic and pet. ether extract. The defeated powdered material was further used for extraction with different organic solvents, namely: aqueous, ethanol, and pet. ether. There was a significant increase in the percentage of radical scavenging activity of ethanolic extract with an increase in concentration, followed by aqueous then pet. ether extracts. But % RSA of ethanolic extract was lesser than that of ascorbic...
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