Posted by admin on Jan 31, 2019 in |
Introduction: Infection due to antimicrobial resistance is currently one of the major threats to mankind. This study investigates the antimicrobial activity of the various extracts of the stem bark of Maesobotrya dusenii (Pax) Hutchinson (Euphorbiaceae). Methods: Pulverized 100 g of stem bark of M. dusenii extracted with methanol. Another 400 g of the plant material was also successively macerated with n-hexane, ethyl acetate, and methanol. These extracts were assessed for antimicrobial activity on clinical isolates of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans using agar diffusion method and minimum inhibitory concentration was determined. Ciprofloxacin was used as a standard antibacterial drug, while ketoconazole was used as a standard antifungal drug. Results: The ethyl acetate extract was the most active extract on E. coli with a minimum inhibitory concentration (MIC) of 0.025 mg/mL. The n-hexane extract also exhibited activity on E. coli with MIC of 0.196 mg/mL. Only n-hexane extract was active against Candida albicans with a MIC of 12.5 mg/mL. Conclusion: The ethyl acetate extract was active...
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Posted by admin on Nov 30, 2018 in |
The present study deals with the chemistry and antifungal activity of essential oil (EO) of two species of Mentha viz. Mentha piperita and Mentha arvensis which was extracted using Clevenger-type apparatus. The percent yield of EO of M. arvensis (2.8%) was found higher than M. piperita (2.1%). Compositional analysis of M. piperita and M. arvensis EO oil by GC-MS revealed the presence of 51 and 35 compounds, respectively. Vibration spectroscopy performed in the 500-4000 cm-1 wave number region depicted variations in the presence of functional groups among the two EOs. The M. piperita EO exhibited better antifungal potential against Penicillium digitatumin comparison to M. arvensis EO. The results were found at par with the standard Carbendazim 50 WP at 250 µg/mL. Optical research microscopy study depicted profound morphological changes in hyphae, and spore-bearing organs of EO treated fungus, thereby supporting the better antifungal activity of M. piperita...
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Posted by admin on Nov 30, 2018 in |
The purpose of this study was to substantiate the use of suspension of Chlorella genus microalgae as the most valuable raw material for the preparation of carotene and chlorophyll а. The method is proposed for ultrasonication of Chlorella vulgaris microalgae suspension to increase the availability of biologically active substances and improve the microbiological performance of the prepared product. Before and after ultrasonication of samples, we determined the content of chlorophylls (a, b, c) and carotenoids using the spectrophotometric method, the level of total bacterial contamination using the method of limiting dilutions on MacConkey agar and microscopic study of microalgae and bacteria cell viability. It has been established that the ultrasonication method at wave amplitude 16 µm allows to transfer pigments into the water phase, inhibit the viability of bacteria and obtain homogenization of the Chlorella vulgaris microalgae suspension with preservation of carotenoids and green chlorophyll а. Thus, the combined use of ultrasonication and parallel plating on MacConkey agar seems to be promising given preparation and quality control of axenic...
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Posted by admin on Nov 30, 2018 in |
India is well known as the emporium of medicinal plants harboring many plants of medicinal importance, of which Curculios orchioides is one that has been reported to possess medicinal importance for the treatment of various diseases traditionally as well as scientifically. An attempt was made herein to undertake a detailed pharmacognostical and preliminary phytochemical investigation of the root tubers of Curculigo orchioides Gaertn. for the first time. The pharmacognostic study comprises of the taxonomical characters of the root tubers of Curculigo orchioides, histochemical tests, and diagnostic characters. The phytochemical analysis consists of the determination of physicochemical constants, fluorescence analysis, and preliminary organic analysis. The diagnostic characters of the plant showed that the leaves are radical with parallel venation while flowers are solitary, trimerous, and ovary inferior. These parameters help in the standardization of the crude drug and in formulating Pharmacopoeial standards for the crude drug. Preliminary phytochemical screening of the crude drug showed the presence of carbohydrates and glycosides, phytosterols, fixed oils and fats, phenolic compounds and tannins, saponins,...
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Posted by admin on Oct 31, 2018 in |
Aim of study: Compare the study on physio-chemical parameters of Acacia arabica and Prosopis juliflora. Material and Methods: The ethanolic extract of on Acacia arabica and Prosopis juliflora were using physio-chemical parameters and preliminary phytochemical investigation. Results and Discussion: In this study. I have done the comparative pharmacognostic study on Acacia arabia and Prosopis juliflora and conclude that the ethanolic extract of Acacia arabica plays a more significant role and has more significant value than the extract of Prosopis juliflora. Conclusion: The present study was aimed at the pharmacognostical study. Plants Acacia arabica and Prosopis juliflora were studies for pharmacognostical characteristic, namely, morphology, microscopy, which can be of utilized in identification and authentication of the...
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