BIOACTIVE COMPOUND IDENTIFICATION, PHYTOCHEMICAL ESTIMATION, IN-VITRO ANTI-INFLAMMATORY AND ANTIOXIDANT ACTIVITY OF PUPALIA LAPPACEAAbstract
Pupalia lappacea Juss belongs to the family Amaranthaceae useful in the bone fracture treatment, antimalarial, pyrexia, wounds, cough, toothache and boils. The present investigation was aimed to identify the phytocompounds by GC-MS, investigate phytochemically and to screen anti-inflammatory and antioxidant property by in-vitro methods. Bioactive compounds were identified using GC- MS; functional groups were identified using FT-IR, phytochemical estimation was done for the phenolics, flavonoids, tannins, and alkaloids, in-vitro anti-inflammatory activity by heat-induced hemolytic and albumin denaturation method, in-vitro antioxidant by DPPH, SOD, NO, PPO and CAT methods. GC-MS analysis of the methanolic extract confirmed the presence of bioactive principles like phenols, tetradecanoic acid, pentadecanoic acid, Eicosatetraenoic acid, Hexatriacontane, eicosapentaenoic acid, and chloroundecane. Methanolic extract of aerial parts of Pupalia lappacea was estimated for the presence of secondary metabolites and screened for in-vitro anti-inflammatory and antioxidant effects. Aerial parts were extracted and estimated for total phenolics 248.19 ± 4.84, flavonoids 123.47 ± 0.19, tannins 57.12 ± 2.45 and alkaloids 69.29 ± 1.84 quantitatively reaction equivalent per gram of the extract. The FT-IR report showed the presence of functional groups like amines, ester, alkyl and other carbonyl groups. Significant RBC membrane stabilisation effect in heat-induced haemolysis method and egg albumin protein inhibition was studied for in-vitro anti-inflammatory action. The phytochemicals like alkaloids, phenolics, flavonoids and tannins present in the extract might be responsible for the desired anti-inflammatory effect of the methanolic extract, which was confirmed by the preliminary phytochemical tests and by estimation. Also, the extract showed anti-oxidant properties in DPPH, Superoxide dismutase and nitrogen oxide, polyphenol oxidase, and catalase methods compared with the standard ascorbic acid.
A. T. Selvan *, N. S. Subramanian, M. Ramadevi , B. S. G. Prasad and S. K. Muthu
Department of Pharmacology , Teegala Krishna Reddy College of Pharmacy, Meerpet, Saroor Nagar, Hyderabad, Andhra Pradesh, India.
15 July 2014
21 August 2014
28 August 2014
01 September 2014