Posted by admin on Jan 11, 2018 in |
Bacillus cereus is a gram-positive, rod-shaped, motile, beta hemolytic bacteria. These bacteria cause foodborne illness, severe nausea, vomiting, diarrhea, and keratitis. Foodborne illness caused by Bacillus cereus occurs because of the bacterial endospores survival in improperly cooked food. The bacteria produce enterotoxins having high resistance against heat and acids, and they cause the diarrheal and emetic syndrome. The diarrhetic syndromes observed in patients are thought to stem from the three toxins: hemolysin BL (Hbl), non-hemolytic enterotoxin (Nhe) and cytotoxin K (CytK). These enterotoxins are all produced in the small intestine of the host, thus thwarting digestion by endogenous host enzymes. The effect is the loss of cellular membrane potential and eventually cell death. Antibiotics that are used for the treatment of infectious disease have suffered a major setback due to the development of drug resistance by the pathogenic target organism. Therefore, in the current study, the riboswitches are explored as an alternative potential drug target. A riboswitch of Bacillus cereus bacteria has been identified as a drug target, and...
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Posted by admin on Jan 11, 2018 in |
The study aims to standardize a vaginal herbal formulation consisting of stem barks of Ficus glomerata Roxb. and Symplocos racemosa Roxb. Since they are used as Ayurvedic medicine and described in ancient text Charak Samhita as astringent, anti-inflammatory and treatment of vaginitis/leucorrhoea. This new herbal formulation is likely to exhibit significant antimicrobial action due to the presence of phenolic and flavonoid compounds. The extractive value was 1.64 and 1.70% w/w for aqueous and alcoholic extract. Total Phenol Content was 225.67 and 220.00 µg Gallic acid equivalent/mg for aqueous and alcoholic extract. Fifteen types of mucoadhesive tablets weighing 550, 700 or 900 mg each were prepared using combinations of research drug, Carbopol and Hydroxy-Propyl-Methyl Cellulose as polymers, talc as a glidant, magnesium stearate as a lubricant and Micro-Crystalline Cellulose and Lactose monohydrate as diluents. Similarly, five types of effervescent mucoadhesive tablets using sodium bicarbonate and citric acid in 3:1 ratio were prepared. Apart from weight, thickness, hardness, and friability of each group, the surface pH, swelling index and muco-adhesive force...
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Posted by admin on Jan 11, 2018 in |
Objective: To evaluate the chemical composition, antioxidant and antiproliferative activities of Hyptis pectinata aerial part aqueous extract (HYP). Method: Chemical profiling using high-performance liquid chromatography with UV diode array detector (HPLC-UV-DAD), in-vitro antioxidant and anti-proliferative studies were done. Antioxidant and antiradical activities of HYP was investigated using 3-(4,5-dimethylimidazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and DPPH radical scavenging assay. Result: The present study revealed several metabolites. HPLC analysis gave caffeic acid (13.92%), rutin (7.89%) and ferulic acid (5.44%) as some of the bioactive constituents. HYP showed antioxidant activity in both the DPPH and MTT assay. HYP exhibited antioxidant activity against DPPH and MTT from the first dilution at 10 mg/ml up to the ninth dilution at 0.08 mg/ml in a 2-fold dilution signifying the presence of antioxidant compounds at those concentrations. At 1 mg/ml to 32 mg/ml, HYP dose-dependently and significantly (P < 0.001) inhibited Sorghum bicolor seed radicle growth throughout 24-72 h compared to the negative control. Conclusion: Hyptis pectinata aerial part aqueous extract (HYP) possessed antioxidant and antiproliferative activities, hence providing...
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Posted by admin on Jan 11, 2018 in |
Diabetes mellitus (DM) is being one of the major causes of morbidity and mortality, which seriously threatens the health of human beings. Worldwide around 366 million people are found to be affected by diabetes. DM is a chronic condition associated with abnormally high levels of sugar (glucose) in the blood. Absence or insufficient production of insulin, or an inability of the body to properly use insulin causes diabetes. DM can be classified as either type 1 or type 2. Type 1 diabetes conditions or insulin-dependent, which treated by insulin therapy. Type 2 diabetes or non-insulin-dependent, which treated by oral hypoglycemic drugs. Conventional insulin and other oral anti-diabetic agents delivery associated with many drawbacks, the need for frequent monitoring of blood glucose, multiple insulin injections, and adjustment of insulin dosages, self-injection, and difficulties in using the vial and syringe technique are among the barriers to patient compliance. Most oral anti-diabetic drugs exhibit low oral bioavailability and need frequent dosing owing to short half-lives, resulting in poor patient compliance. Therefore, to...
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Posted by admin on Jan 28, 2017 in |
Loganin is an iridoid glycoside, a key phytoconstituent in Strychnos plants having a crucial role in the biosynthesis of indole, monoterpene alkaloids and other natural products. It is a bitter iridoid glycoside with numerous activities like anti-inflammatory, anti-oxidant, glucose-lowering and anti-diabetic nephropathy activities. The objective of this study was to develop a simple method for isolation of loganin from fruit pulp of Nux vomica and quantify by HPLC. The isolated compound was characterized by TLC, UV, IR, MS, and NMR spectral analysis and purity were confirmed by HPLC analysis. Quantification of loganin was done from fruit, seeds and pulp extract using HPLC. A simple solvent partitioning method was used for the extraction and isolation process. This isolation method developed involved easy solvent-solvent extraction technique followed by recrystallization to purify the...
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