EVALUATION OF THE ANTIOXIDANT AND ANTI-PROLIFERATIVE CHEMICAL CONSTITUENTS OF HYPTIS PECTINATA (LINN.) AERIAL INFUSIONAbstract
Objective: To evaluate the chemical composition, antioxidant and antiproliferative activities of Hyptis pectinata aerial part aqueous extract (HYP). Method: Chemical profiling using high-performance liquid chromatography with UV diode array detector (HPLC-UV-DAD), in-vitro antioxidant and anti-proliferative studies were done. Antioxidant and antiradical activities of HYP was investigated using 3-(4,5-dimethylimidazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and DPPH radical scavenging assay. Result: The present study revealed several metabolites. HPLC analysis gave caffeic acid (13.92%), rutin (7.89%) and ferulic acid (5.44%) as some of the bioactive constituents. HYP showed antioxidant activity in both the DPPH and MTT assay. HYP exhibited antioxidant activity against DPPH and MTT from the first dilution at 10 mg/ml up to the ninth dilution at 0.08 mg/ml in a 2-fold dilution signifying the presence of antioxidant compounds at those concentrations. At 1 mg/ml to 32 mg/ml, HYP dose-dependently and significantly (P < 0.001) inhibited Sorghum bicolor seed radicle growth throughout 24-72 h compared to the negative control. Conclusion: Hyptis pectinata aerial part aqueous extract (HYP) possessed antioxidant and antiproliferative activities, hence providing preliminary evidence for its use to treat cancer.
S. E. Okhale *, S. S. Ode, P. Oladosu and G. E. Ugbabe
Department of Medicinal Plant Research and Traditional Medicine, National Institute for Pharmaceutical Research and Development, Abuja, Nigeria.
13 May 2017
22 August 2017
16 September 2017
01 January 2018