Posted by admin on Mar 15, 2018 in |
This study was aimed to maximize the benefits of the use of by-products (oil cake) of olive fruits and black seeds after extraction of their fixed oils, rather than their use as animals feed or landfilling or composting. The cake of both seeds was assessed by HPLC for their main bioactive marker compounds (thymoquinone for black seed and oleuropein for olive fruit), to choose the best method for extraction, determination of phenolic contents and in vitro antioxidant activity (DPPH and reducing powers, FRAP assays). Both by-products were also assessed for their cytotoxicity against four human breast cell lines, three of them are cancerous (MCF7, MDA-MB-213, Vcr-R), and a non-cancerous one (epithelial type) but immortalized by telomerase (hTERT-HME), in addition to a human hepatoma cell line (HepG2). Also, both wastes were subjected for in-vitro CDC25s phosphatase inhibition assay on three isoforms (A-C) and for in-vivo immunomodulatory effects. In conclusion, the results of this study showed the interest of cumulating different biological approaches exploring various physiological mechanisms and showed the utility...
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Posted by admin on May 17, 2016 in |
The hypolipidemic activity of Aloe vera (Family: Liliaceae) extract has been studied in two models of hyperlipidemia in rats. In an acute model, hyperlipidemia was induced by injecting a single dose of triton WR-1339 (400mg/kg, b.w.) intraperitoneally in rats. Feeding with Aloe vera extract at the dose of 500 mg/ kg, b.w. exerted a significant lipid-lowering effect as assessed by the reversal of plasma levels of total cholesterol (TC), phospholipids (PL), triglyceride (TG) and reactivation of post-heparin lipolytic activity (PHLA). In the chronic model, hyperlipidemia was induced by feeding with cholesterol-rich- HFD in rats. The treatment with seeds extracts of Aloe vera (500 mg/ kg b.w) simultaneously for 15 days also caused lowering of lipid levels in plasma and liver following reactivation of plasma post-heparin lipolytic activity and hepatic lipoprotein lipase activity in animals. The hypolipidemic activity of Aloe vera was compared with a standard drug guggulipid (200 mg/ kg, b.w.) in both...
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Posted by admin on Dec 1, 2014 in |
Amino acids are biologically active substances, and a number of them are essential for living beings. Amino acids are found in living cells as well as in body fluids of higher animals, in amounts, which vary according to the tissue and particular amino acid. Amino acid analysis refers to the methodology used to determine the amino acid composition or content of proteins, peptides, and other pharmaceutical preparations. Proteins and peptides are macromolecules consisting of covalently bonded amino acid residues organized as a linear polymer. The sequence of the amino acids in a protein or peptide determines the properties of the molecule. Proteins are considered large molecules that commonly exist as folded structures with a specific conformation, while peptides are smaller and may consist of only a few amino acids. Amino acid analysis can be used to quantify protein and peptides, to determine the identity of proteins or peptides based on their amino acid composition, to support protein and peptide structure analysis, to evaluate fragmentation strategies for peptide mapping, and...
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Posted by admin on Sep 1, 2014 in |
Amino acids are biologically active substances, and a number of them are essential for living beings. Amino acids are found in living cells as well as in body fluids of higher animals, in amounts, which vary according to the tissue and particular amino acid. Amino acid analysis refers to the methodology used to determine the amino acid composition or content of proteins, peptides, and other pharmaceutical preparations. Proteins and peptides are macromolecules consisting of covalently bonded amino acid residues organized as a linear polymer. The sequence of the amino acids in a protein or peptide determines the properties of the molecule. Proteins are considered large molecules that commonly exist as folded structures with a specific conformation, while peptides are smaller and may consist of only a few amino acids. Amino acid analysis can be used to quantify protein and peptides, to determine the identity of proteins or peptides based on their amino acid composition, to support protein and peptide structure analysis, to evaluate fragmentation strategies for peptide mapping, and...
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Posted by admin on Apr 1, 2014 in |
The poisonous and medicinal plants are found in a natural ecosystem. The Arisaema murrayi (Hook) is an herb which is highly poisonous. It is found in the hilly region of Mumbai area. Livestock, cattle and human beings do not consume fruits and tubers of the plant, so the forensic study of this plant is carried out. Aqueous extracts of tuber 2%, 4% and 6% feed orally to Swiss albino mice. Present investigation study includes the histopathology, phyto-chemistry and quantitative microscopy. It is observed that liver, intestine, and kidney were severally damaged by using the Arisaema murrayi (Hook) plant parts. The forensic study proved that these organs were damaged by the poison of Arisaema murrayi (Hook) plant of the...
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