VALORIZATION OF EGYPTIAN FOOD BYPRODUCTS IN THE DEVELOPMENT OF BIOLOGICALLY ACTIVE NUTRACEUTICALSAbstract
This study was aimed to maximize the benefits of the use of by-products (oil cake) of olive fruits and black seeds after extraction of their fixed oils, rather than their use as animals feed or landfilling or composting. The cake of both seeds was assessed by HPLC for their main bioactive marker compounds (thymoquinone for black seed and oleuropein for olive fruit), to choose the best method for extraction, determination of phenolic contents and in vitro antioxidant activity (DPPH and reducing powers, FRAP assays). Both by-products were also assessed for their cytotoxicity against four human breast cell lines, three of them are cancerous (MCF7, MDA-MB-213, Vcr-R), and a non-cancerous one (epithelial type) but immortalized by telomerase (hTERT-HME), in addition to a human hepatoma cell line (HepG2). Also, both wastes were subjected for in-vitro CDC25s phosphatase inhibition assay on three isoforms (A-C) and for in-vivo immunomodulatory effects. In conclusion, the results of this study showed the interest of cumulating different biological approaches exploring various physiological mechanisms and showed the utility of these extracts in different fields, the first being used as a cytoprotective agent, and the second one being promising as an anticancer agent.
R. S. El Dine, T. Tzanova, D. Bousta, D. R. Abou-Hussein, S. Boukhira, E. Evain-Bana, N. D. El-Tanbouly, S. Philippot, L. El Mansourai, D. Bagrel, R.I. Amer and E. Abdel-Sattar *
Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
23 October 2017
12 November 2017
18 November 2017
01 March 2018