UV-VISIBLE SPECTROPHOTOMETRIC ESTIMATION OF CURCUMIN IN NANOFORMULATIONAbstract
A rapid, simple, selective and precise UV-Visible spectrophotometric method has been developed for the determination of Curcumin in bulk and nano-formulation. The spectrophotometric detection was carried out at an absorption maximum of 421 nm using methanol as solvent. The method was validated for various validation parameters. The linearity range was found to be 5-25 μg/ml with a correlation coefficient of 0.9997. The accuracy was found to be within the limit. The LOD and LOQ were found to be 0.4 and 1.21 μg/ml; respectively. The results demonstrated that the method could be conveniently employed for routine quality control analysis of Curcumin in bulk and formulations.
K. Hazra *, R. Kumar, B. K. Sarkar, Y. A. Chowdary, M. Devgan and M. Ramaiah
National Institute of Ayurvedic Pharmaceutical Research, Patiala, Punjab, India.
27 December 2014
17 January 2015
27 February 2015
01 March 2015