STUDY OF PHYTOCHEMICAL ANALYSIS AND ANTIOXIDANT ACTIVITY OF SPINACH OLERACEA L PLANT LEAVESAbstract
Spinach is a leafy green flowering plant. The leaves are a common edible vegetable consumed by fresh leaves. The bioactive components present in the Spinach oleracea plant leaves are known to be responsible for its medicinal properties. The present study was undertaken to compare the effect of different extraction solvents to extract the active components like carbohydrates, tannin, flavonoid, phenol, carbohydrate from the dried leaves of Spinach oleracea plant. The extraction solvents used were Ethyl acetate, Petroleum ether and Methanol. Phytochemical analysis, total flavonoid concentration, total phenol concentration and antioxidant activity have been identified to compare the efficiency of different extraction solvents. The results shows that using methanol as an extraction solvent works best for the extraction of various active phytochemicals, the total flavonoid and phenols concentration and the antoxidant activity showed the effective scavenging activity. This is the report that directly compares the three extraction solvents for the extraction of active components from the Spinach oleracea leaves and shows that method should be the solvent of choice.