SPERMICIDAL ACTIVITY OF ELETTARIA CARDAMOMUM AND CUMINUM CYMINUM SEED EXTRACTS AND ASSESSMENT OF SPERM FUNCTION IN ALBINO RATSAbstract
The spermicidal activity of Elettaria cardamomum and Cuminum cyminum seed extracts have been studied to evaluate the effective concentration to immobilize and kill 100% of sperm within 20 s, and the sperm function of rat is studied to find the effect on sperm membrane integrity. Sander and Cramer’s test was used to study the spermicidal activity of Elettaria cardamomum and Cuminum cyminum. Sperm viability and sperm morphology were studied using Eosin-Nigrosin staining technique. Stability of the extracts was studied by storage of extracts at –20 °C. Under the test conditions, minimum effective spermicidal concentrations for Elettaria cardamomum and Cuminum cyminum seeds extract are 1 mg/million sperm and 5 mg/million sperm respectively. The effect of the extract on morphology and viability of sperm was also studied, and no change was observed in the morphology of head, midpiece, and tail and no viable sperm seen. The effect of different concentrations of extracts of Elettaria cardamomum and Cuminum cyminum on percentage motility of the sperm was also studied. With an increase in concentration, there is a linear decrease in percentage motility, becoming zero at 1mg and 5 mg dose respectively within 20 s. The effectiveness of lyophilized aqueous extract of cardamom and cumin seed extract does not change with time after storage in the deep fridge at -20 ºC for 4 months.
B. Khillare* and A. R. Singh
Department of Reproductive Biomedicine, National Institute of Health and Family Welfare Munirka, New Delhi, Delhi, India
12 January 2014
27 February 2014
30 March 2014
01 April 2014