PROTECTION OF HIPPOCAMPAL AND ISLET BETA CELLS IN VITRO BY EMODIN FROM LEAVES OF RUMEX CONFERTUSAbstract
The natural anthracene derivative emodin (1,3,8-trihydroxy-6-methylanthraquinone) is known as an anticancer agent. In the present work, it was isolated for the first time from the ethanol extract of Rumex confertus leaves, widely used in Armenia as a vegetable. It was purified in two-step low-pressure liquid chromatography – a significantly easier and inexpensive procedure compared with those used for emodin purification. The purified preparation was characterized by chemical and TLC analyses, NMR, UV-Vis and fluorescence spectra. Rather high cytotoxicity toward cultivated primary cells of mice Ehrlich ascites carcinoma was demonstrated for both the ethanol extract (IC50 = 0.3 ± 0.04 µg/ml), and emodin (IC50 = 40 ± 10 ng/ml) from Rumex confertus leaves. Along with the anticancer activity, these preparations protected a) hippocampal cells against toxic action of aggregated amyloid Aβ (1-40) and Aβ (1-42) peptides and b) the islet β-cells against death in the presence of aggregated pancreas peptide hormone amylin. The obtained results provide a rationale for developing anticancer, neuroprotective and antidiabetic remedies from the leaves of Rumex confertus.
A. Antonyan *, S. Sharoyan, H. Harutyunyan, L. Barboni, G. Lupidi and S. Mardanyan
H. Buniatyan Institute of Biochemistry of Armenian National Academy of Sciences, Republic of Armenia.
02 September 2016
05 October 2016
26 October 2016
31 October 2016