PHYTOCHEMICAL SCREENING AND ANTIOXIDANT ACTIVITY OF METHANOLIC ROOT EXTRACT OF ANNONA GLABRA LINN.
AbstractAntioxidants are crucial in combating oxidative stress and related diseases. This study aimed to assess the antioxidant potential of methanolic root extract of Annona glabra Linn. and ascorbic acid is used as a reference. The plant material was subjected to sequential extraction using a solvent of varying polarities, utilizing a Soxhlet extraction method. The extract was tested for phytochemicals (secondary metabolites), total phenolic content (TPC), total flavonoid content (TFC) and antioxidant activity. The phytochemicals examination of root extract of Annona glabra Linn. exposed the presence of alkaloids, glycosides, flavonoids, saponins, phenols, steroids, terpenoids. TPC, TFC of crude methanolic root extract of plant was determined using Folin-Ciocalteus reagent and Aluminum chloride colorimetric method respectively. Antioxidant activities were determined spectrophotometrically using the DPPH assay, nitric oxide scavenging assay, hydrogen peroxide scavenging assay, phosphomolybdate assay, ferric reducing antioxidant power assay. The result of methanolic root extract shows the good antioxidant potency.
Article Information
6
617-626
2309 KB
58
English
IJP
R. Sinchana *, Tamizh T. Mani and L. Shiju
Department of Pharmacognosy, Bharathi College of Pharmacy, Bharathinagara, Mandya, Karnataka, India.
sinchanagowda802@gmail.com
26 October 2024
22 November 2024
26 November 2024
10.13040/IJPSR.0975-8232.IJP.11(11).617-26
30 November 2024
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