PHARMACOGNOSTIC DETERMINATION OF GLYCOSMIS PENTAPHYLLA (RETZ.) DC. FOR TAXONOMIC IDENTIFICATION AND QUALITY CONTROL OF CRUDE DRUGAbstract
The present work has been done with an objective to analyze the crude drug parametrs responsible for tenable antioxidant effect of leaf, stem and root of Glycosmis pentaphylla (Retz.) DC. Fresh leaf, stem and root of Glycosmis pentaphylla were extracted with ethanol (EEGPle, EEGPst, EEGPro). The antioxidant profiling was done through different methods like 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay, 2,2-azino-bis-3-ethyl benzothiazoline acid (ABTS) free radical scavenging assay and nitric oxide (NO) free radical scavenging assay. Crude drug parameters were focussed mainly on micrometric properties so as to determine the flow properties when consumed for medicinal purposes. A significant antioxidant activity was revealed by the EEGPle [EC50: (29.04±0.008) µg/mL], compared to EEGPst and EEGPro in DPPH assay. The ranking order for ABTS was EEGPle > EEGPst > EEGPro. The EC50 value of NO of the EEGPle, EEGPst and EEGPro were (263.14±0.05) µg/mL, (312.62±0.07) µg/mL and (384.62±0.006)µg/mL respectively. Quantity of TFC and TPC were highest in EEGPle (112.96±3.89 mg QRE/g and 96.6±1.08 mg GAE/g extract) rather than EEGPst and EEGPro extracts. The present work suggests that EEGPle has a significantly higher antioxidant property than EEGPst and EEGPro. These extract can help in prevent or slow down the occurrence of different diseases related to oxidative stress. But, a detailed analysis of these extracts is required to determine the presence of promising compound(s) responsible for their antioxidant activity. Better quality control parameters in pharmaceutical industries helps to prevent the adulteration, if any. Hence, this work will help in identiﬁcation and quality control of G. pentaphylla as a medicinal material.
V. S. Babu * and P. M. Radhamany
Plant Reproductive Biology Laboratory, Department of Botany, University of Kerala, Thiruvananthapuram, Kerala, India.
12 April 2020
23 June 2020
27 June 2020
01 July 2020