PHARMACOGNOSTIC AND IN-VITRO ANTIOXIDANT, ANTI-INFLAMMATORY & ANTIDIABETIC ACTIVITY OF THE FLOWER OF THE HIMALAYAN RHODODENDRON ARBOREUM

Objective: To investigate the Pharmacognostic and in-vitro Antioxidant, Anti-inflammatory & Anti-diabetic Activity of the flower of the Himalayan Rhododendron Arboreum. Methods: The collected plant flowers of Rhododendron Arboreum were cleaned with distilled water and air-dried at room temperature under shade (25-27 °C) and reduced to the appropriate size of the powder. The powdered plant materials are further extracted by cold maceration with hydroalcoholic as well as the aqueous solvent. The filtrates obtained from the successive maceration were concentrated under reduced pressure using a rotary evaporator followed by hot air set at 40 °C. The dry extract obtained was harvested, and the dried extract was transferred into airtight bottles and stored in a refrigerator at -4 °C. The powdered flowers of Rhododendron Arboreum and hydroalcoholic and aqueous extract were standardized via pharmacognostic parameters. Phytochemical screening of Hydro-alcoholic and aqueous extract of Rhododendron Arboreum revealed the presence of tannins, flavonoids, phenol, triterpenes, Steroids & carbohydrates. Different flowers extract of Rhododendron Arboreum accessed for in vitro antioxidant potential by using the DPPH assay and NO Scavenging assay by using ascorbic acid as a standard drug. Further, the Hydro-alcoholic and aqueous extract of Rhododendron Arboreum flower are to be subjected for the assessment of its anti-inflammatory potential by use of Heat-induced hemolysis as well as Hypotonicity-induced hemolysis approach by the use of the aspirin or diclofenac sodium as a standard drug. Further, the Different flowers extract of Rhododendron Arboreum is accessed for its antidiabetic potential by using the in-vitro alpha-amylase inhibition and alpha-glucosidase inhibition approach by using the Acrabose as standard drug. Results: Phytochemical screening of Hydro-alcoholic and aqueous extract of Rhododendron Arboreum flower revealed the presence of tannins, flavonoids, phenol, triterpenes, Steroids & carbohydrates. In-vitro study shows that the Hydro-alcoholic and aqueous extract of Rhododendron Arboreum flower has excellent antioxidant, anti-inflammatory, and antidiabetic potential in a dose-dependent manner compared to the result standard drugs. Conclusion: From this investigation, it is to be suggested that the Hydro-alcoholic and aqueous extract of Rhododendron Arboreum flower is rich in the phenolic & tannin compound. The experimentation study shows the drug is to possesses good antioxidant anti-inflammatory as well as antidiabetic properties.