NEUROPROTECTIVE ACTIVITY OF ASCOPHYLLUM NODOSUM ON EXPERIMENTLLY INDUCED GLOBAL CEREBRAL ISCHEMIA/REPERFUSION INJURY IN MICE

Background: Ascophyllum nodosum has been used in oriental medicine to treat a variety of remedies, including some neurological disorders. Ascophyllum nodosum rich in phenolic antioxidant (rich in polyphenol) have been identified as major active component exhibiting antioxidant, anti-inflammatory and neuroprotective effects. The present study is to evaluate the neuroprotective effect of Ascophyllum nodosum on experimentally induced Global Cerebral Ischemia/Reperfusion Injury in mice. Material and Methods: Neuroprotective activity was carried out by global cerebral ischemia on Swiss albino mice by carotid artery occlusion for 17 min followed by 24 h reperfusion. Then after 24 h blood sample was collected by retro-orbital route and after that mouse was sacrificed by cervical dislocation under light area, the brain was removed for various biochemical analyses. Results: Ascophyllum nodosum hydroalcholic extract showed dose-dependent neuroprotective activity by a significant decrease in lipid peroxidation (LPO), lactate dehydrogenase (LDH) and serum nitrite level in extract treated the group as compared to the ischemia/reperfusion group. Cerebral infraction area was significantly reduced in extract treated groups as compared to ischemia/reperfusion group. Conclusion: In the present investigation, a polyphenolic fraction of A. nodosum has shown promise as a Cerebral Ischemia/Reperfusion Injury curing, as well as enhancing agents in mice in the entire laboratory model employed. Furthermore, the polyphenolic extract of A. nodosum was found more effective than against global Cerebral Ischemia/Reperfusion Injury.