ISOLATION AND CHARACTERIZATION OF ACTIVE CONSTITUENTS FROM PLANT PISONIA ACULEATA LINN BY SPECTRAL ANALYSIS

Botanicals and herbal preparations for medicinal usage contain various types of bioactive compounds. The focus of this paper is on the analysis of bioactive compounds present in the Pisonea aculeata Linn. having anticancer activity involving the applications of chromatographic techniques such as TLC and column chromatography, Spectrophotometric techniques such as UV, ¹H-NMR, ¹³C-NMR, and EIMS spectral studies. The present investigation was carried out by fractionating the Methanolic extract of Pisonia aculeata (MPA) using benzene, diethyl ether, and ethyl acetate. Ethyl acetate fraction on concentration subjected to separation and purification on column chromatography. Fractions were collected & homogeneity was examined on TLC. A Fraction 77-90 on concentration yielded a pure yellowish homogeneous solid and gave dark green coloration with neutral ferric chloride and violet coloration with Molish’s reagent. In order to find out the nature of the glycoside, compound I was subjected to acid hydrolysis. The basic flavonoid structure of the compound studied by recording UV spectrophotometer showed an intense UV maxima at 273 nm (band II) and 329 nm (band I), indicating the flavone nature of it. The ¹H-NMR spectrum of compound I was recorded using AMX 400 (400 MHz) spectrometer using DMSO-d6 as the solvent showed a pair of doublets in the aromatic region at d 7.89 ppm and d 6.93 ppm.  Electron Impact – Mass Spectrometry (EI-MS) of the compound exhibited M+ at m/z 330. Thus based on the R_f values, UV, ¹H-NMR, ¹³C-NMR, and EIMS spectral studies, the structure of the compound has identified as 5, 4¢-Dihydroxy 6,8-dimethoxy 7-O-rhamnosyl flavones.