INVESTIGATION ON NOVEL ROLE OF SOLANUM XANTHOCARPUM AND JUNIPERUS COMMUNIS EXTRACT AGAINST CCl4 INDUCED LIVER INJURYAbstract
Background: There are very less therapeutic and reliable liver protective drugs in modern medicine to prevent and treat the liver injury caused due to drugs. Traditionally Solanum xanthocarpum Schradt. (SX) and Wendl. and Juniperus communis Linn. (JC) have been used since long for their hepatoprotective effect. The current study was carried out to explore the hepatoprotective role of SX and JC against liver toxicity in rodents induced by carbon tetrachloride (CCl4). Materials and Methods: The antioxidant potential of hydroalcoholic extract of SX and JC were investigated by using established DPPH in-vitro assay method. The hydroalcoholic extract of SX and JC at doses (200 and 400 mg/kg b.w.) were studied for their liver protective activity against hepatic damage induced by CCl4 in Wistar rats. An assay of the oxidative stress parameters, alkaline phosphatase, transaminase, total bilirubin, albumin and liver histopathology were performed to evaluate the hepatoprotective activity of SX and JC. The assay results were introduced as the standard error ofthe mean (SEM) for each group. Results: The ethanolic extract of SX and JC has shown the potent antioxidant scavenging in-vitro activity viz. DPPH IC50 of 69.41 ± 0.76 µg/ml, 117 ± 0.34 µg/ml respectively. The hydroalcoholic extract of SX and JC showed a significant reduction in oxidative stress parameters and improved antioxidant and liver enzymes level as compare to toxicant treated rats. Moreover, histopathological studies also revealed the similar results which supported the liver protective activity of SX and JC herbal extracts. Conclusion: It is concluded that SX and JC showed significant hepatoprotective activity in CCl4 induced hepatoxicity in rodents. The promising mechanism for their therapeutic activity is due to their antioxidant and liver protective activity which scientifically supports their traditional use.
B. C. Joshi *, S. Uniyal and Sukanya
Department of Pharmacognosy, Sardar Bhagwan Singh Post Graduate Institute of Biomedical Sciences and Research, Balawala, Dehradun, Uttarakhand, India.
24 June 2017
07 July 2017
17 September 2017
01 December 2017