INVESTIGATING THE ANTIOXIDANT PROPERTIES AND CHEMICAL COMPOSITION OF LINUM USITATISSIMUM L. (FLAXSEED) EXTRACT USING HR-LCMS ANALYSIS

This study investigates the antioxidant properties and chemical composition of Linum usitatissimum L. (flaxseed) extract using High Resolution-Liquid Chromatography Mass Spectrometry (HR-LCMS) analysis. The primary objective is to identify the bioactive compounds responsible for the antioxidant activity and evaluate the efficacy of flaxseed extracts prepared with different solvents, including methanol, ethanol, acetone, chloroform, petroleum ether, and ethyl acetate. Methodologically, the study employs various antioxidant assays such as DPPH, ABTS, FRAP, and total Antioxidant Activity (TAA), in conjunction with HR-LCMS analysis for comprehensive chemical profiling. Key findings reveal that methanolic and ethanolic extracts exhibit the highest antioxidant activities, with significant amounts of phenolic and flavonoid compounds identified. The HR-LCMS analysis further elucidates the presence of metabolites such as adenosine, abscisic acid glucose ester, and kaempferol 7-O-glucoside, which contribute to the observed bioactivity. The study concludes that flaxseed extracts, particularly those obtained with methanol and ethanol, possess substantial antioxidant potential, underscoring their potential application in nutraceuticals and functional foods.