IN VITRO FREE RADICAL SCAVENGING ACTIVITY OF APOROSA LINDLEYANA BAILL.Abstract
The free radical scavenging activity of ethanolic root extract of Aporosa lindleyana (EREAL) was evaluated in-vitro with the spectrophotometric method based on the inhibition of hydroxyl radical, the reduction of stable DPPH free radical, inhibition of superoxide anion radical, total antioxidant activity and reducing power. Preliminary phytochemical screening showed the presence of phenolic compounds and flavonoids. EREAL showed significant activities in all free radical scavenging assays compared to standard antioxidants such as ascorbic acid, butylated hydroxyl toluene and vitamin E. In DPPH scavenging assay the IC50 value of EREAL was 215.51 µg/mL, while vitamin E had 299.11 µg/mL. Furthermore, EREAL showed good free radical scavenging activity on reducing power, superoxide anion radical activity, total antioxidant activity, hydroxyl radical activity and had good reducing power.
S. Ramakrishnan * and R. Venkataraman
Department of Biotechnology, Sri Paramakalyani College, Alwarkurichi, Tamil Nadu India.
12 February 2018
26 March 2018
30 March 2018
01 June 2018