IN-VITRO CYTOTOXICITY OF ASSAY OF LEAVES OF PORTULACA QUADRIFIDA USING BRINE SHRIMP ASSAYAbstract
Portulaca quadrifida Linn. (Portulacaceae) is commonly known as a chicken weed in English. In the traditional system, it is used to treat rheumatism and gynecological diseases, urinary tract infections, worm diseases, dysentery and dermatitis. To assess the cytotoxic potential of hydroalcoholic extract of P. quadrifida leaves using brine shrimp assay. Potassium dichromate was considered a standard reference drug. The concentrations of 10, 100, 1000 μg/ml were used in the experiment. The mature shrimps were taken, and it was treated with the extract and the standard in the respective concentrations. LC50 value was calculated for both standard and extract, and the probit analysis was made by using SPSS software. From the probit analysis, the cytotoxicity of the extract was determined by comparing it with the standard. The graph was plotted for both the standard and the extract; the extract was found to be non- toxic.
R. T. Kumaran * and N. Naidu
Department of Pharmacognosy, Faculty of Pharmacy, Sri Ramachandra Medical College and Research Institute, Porur, Chennai, Tamil Nadu, India.
27 March 2018
12 June 2018
17 June 2018
01 July 2018