IN-VITRO ANTIDIABETIC ACTIVITY OF CHAMAECOSTUS CUSPIDATUS

Background: The most common disease of endocrine disorder is diabetes due to underlying causes and pathophysiologies. It is spreading swiftly worldwide and is expected to reach up to 4.4% in the world by 2030. There are numerous synthetic glucose-lowering drugs on the market, but they all have detrimental effects. As a result, this necessitates the search for an alternative anti-diabetic agent from a natural source, i.e., herbal remedies that have been used traditionally and have a safe profile. Aim of the study: The purpose of this study was to look into the anti-diabetic activity of C. cuspidatus methanolic leaves extract on the glycation of Bovine Serum Albumin by measuring fluorescence intensity at various concentrations (1 mg/ml, 2 mg/ml, 4 mg/ml, 6 mg/ml, 8 mg/ml, 10 mg/ml) using positive control 1mM of Aminoguanidine (1 mg/ml, 2 mg/ml, 4 mg/ml, 6 mg/ml, 8 mg/ml, 10 mg/ml). Methodology: Phytochemical screening of Chamaecostus cuspidatus for determination of Phytoconstituents. BSA- Glucose Assay for in-vitro Glycation- methodology (Brownlee et al., 2013). BSA (l0 mg/mL) was incubated with glucose (500mM) in phosphate-buffered saline (PBS) (5mL total volume, pH 7.4) and extract containing 0.02% sodium azide at 37 °C with a final concentrations of BSA (2mg/mL), glucose (40 mM), sample (0.1 to 0.5mg/mL). All the reagents and samples were sterilized by filtration through 0.2 μm membrane filters. Results: The phytochemical screening shows the presence of flavonoids, saponins, terpenoids, tannins, and chlorophyll. The BSA- Glucose Assay for in-vitro Glycation shows that after 15 days of incubation with methanolic extract of Chamaecostus cuspidatus and aminoguanidine the fluorescence intensity was significantly decreased in a concentration-dependent manner. Conclusion: The reductions in fluorescence intensity reveal that methanolic extract of Chamaecostus cuspidatus can inhibit the formation of AGEs and provide protection from diabetic complications.